| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GPRK5; FP2025 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GRK5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRK5抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*GRK5-mediated desensitization of β-adrenergic signaling in cardiac myocytes*
**作者**：Tilley, D.G., et al.
**摘要**：研究利用GRK5特异性抗体分析其在心肌细胞中的表达及功能，发现GRK5通过磷酸化β-肾上腺素受体介导信号脱敏，参与心力衰竭病理过程。
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2. **文献名称**：*Role of GRK5 in cancer cell migration and invasion*
**作者**：Chen, X., et al.
**摘要**：通过免疫沉淀(使用GRK5抗体)和基因敲除实验，揭示GRK5通过调控细胞骨架重组促进乳腺癌转移，提示其作为潜在治疗靶点。
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3. **文献名称**：*GRK5 deficiency attenuates amyloid-β accumulation in Alzheimer's disease models*
**作者**：Suo, Z., et al.
**摘要**：利用GRK5抗体检测脑组织中的表达水平，发现GRK5缺失可减少Aβ沉积，表明其在阿尔茨海默病中调控淀粉样蛋白代谢的关键作用。
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以上文献展示了GRK5抗体在心脏疾病、癌症及神经退行性疾病研究中的应用，涵盖机制探索与治疗潜力分析。
The G protein-coupled receptor kinase 5 (GRK5) is a member of the GRK family, which regulates the desensitization and signaling of G protein-coupled receptors (GPCRs) by phosphorylating their activated forms. Primarily expressed in the heart, brain, and other tissues, GRK5 plays critical roles in cardiovascular function, neuronal signaling, and cellular proliferation. Dysregulation of GRK5 has been implicated in pathologies such as heart failure, hypertension, Alzheimer’s disease, and cancer, making it a target for therapeutic and diagnostic research.
GRK5 antibodies are essential tools for studying the expression, localization, and functional mechanisms of GRK5 in both physiological and disease contexts. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect GRK5 protein levels in tissues or cultured cells. Researchers also employ GRK5-specific antibodies to investigate its interactions with GPCRs, β-arrestin, and other signaling molecules, as well as its role in pathways like NF-κB activation.
Commercial GRK5 antibodies are typically generated in rabbits or mice using peptide antigens derived from conserved regions of the human GRK5 protein. Validation includes testing for specificity via knockout cell lines or siRNA knockdown. Challenges in antibody performance, such as cross-reactivity with other GRK isoforms, necessitate careful selection and optimization for experimental accuracy. Overall, GRK5 antibodies are vital for advancing insights into GPCR-related diseases and potential drug development.
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