| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | KAT4; KATIV; mitAAT |
| WB Predicted band size | 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GOT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GOT2抗体的3篇参考文献(基于公开信息整理，部分为示例性内容)：
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1. **文献名称**: *"Mitochondrial aspartate aminotransferase (GOT2) regulates cancer cell energy metabolism and tumor growth"*
**作者**: Smith A, et al.
**摘要**: 研究通过Western blot和免疫荧光技术，利用GOT2特异性抗体验证其在多种癌细胞系中的表达，发现GOT2通过调控谷氨酰胺代谢促进肿瘤增殖，靶向GOT2可能成为癌症治疗新策略。
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2. **文献名称**: *"Role of GOT2 in hepatic mitochondrial function and insulin resistance"*
**作者**: Chen L, et al.
**摘要**: 文章使用GOT2抗体进行免疫组化和小鼠肝脏组织分析，证明GOT2缺失导致线粒体功能异常，并加重胰岛素抵抗，揭示了其在代谢疾病中的关键作用。
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3. **文献名称**: *"Characterization of a monoclonal antibody targeting human GOT2 for detecting mitochondrial dysfunction"*
**作者**: Tanaka K, et al.
**摘要**: 研究报道了一种高特异性抗GOT2单克隆抗体的开发，验证其在ELISA和免疫印迹中的灵敏度，并应用于阿尔茨海默病模型中检测线粒体损伤标志物。
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**备注**：以上文献为示例性内容，实际文献需通过PubMed或学术数据库检索确认。建议使用关键词“GOT2/AST2 antibody”或“aspartate aminotransferase mitochondrial antibody”查询最新研究。
The GOT2 antibody targets the mitochondrial enzyme glutamate oxaloacetate transaminase 2 (GOT2), also known as aspartate aminotransferase 2 (AST2). GOT2 is a key player in the malate-aspartate shuttle, facilitating the transfer of reducing equivalents (NADH) across mitochondrial membranes to support cellular energy production. It catalyzes the reversible conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate, linking amino acid metabolism with the tricarboxylic acid (TCA) cycle. Dysregulation of GOT2 has been implicated in metabolic disorders, cancer progression, and mitochondrial dysfunction.
The GOT2 antibody is widely used in research to investigate protein expression, localization, and function in tissues or cell lines. It serves as a critical tool in Western blotting, immunohistochemistry, and immunofluorescence to study metabolic adaptations in diseases such as hepatocellular carcinoma, renal pathologies, and neurodegenerative conditions. Recent studies highlight its role in tumor microenvironment remodeling, where GOT2 modulates redox balance and nucleotide synthesis. Commercial GOT2 antibodies are typically raised against specific epitopes, with validation in knockout models to ensure specificity. Researchers also explore its potential as a diagnostic or prognostic biomarker, given its altered expression in certain cancers. Understanding GOT2 dynamics through antibody-based assays continues to advance insights into mitochondrial metabolism and therapeutic targeting strategies.
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