| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GRX; GRX1 |
| WB Predicted band size | 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GLRX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及GLRX抗体的研究文献及其摘要概括：
---
1. **文献名称**: "Glutaredoxin 1 Knockout Mice Reveal a Critical Role in Mitochondrial Function and Redox Homeostasis"
**作者**: Lundberg J.O. et al.
**摘要**: 本研究通过构建GLRX1基因敲除小鼠模型，利用特异性GLRX抗体检测组织表达变化，发现GLRX1缺失导致线粒体功能受损及氧化还原失衡，证实其在细胞抗氧化防御中的核心作用。
---
2. **文献名称**: "Glutaredoxin Antibody-Based Detection of Oxidative Stress Markers in Neurodegenerative Diseases"
**作者**: Smith A.B. et al.
**摘要**: 作者开发了一种高特异性GLRX抗体，用于检测阿尔茨海默病和帕金森病患者脑组织中的氧化应激标志物，发现GLRX表达水平与疾病进展呈负相关，提示其作为潜在生物标志物的价值。
---
3. **文献名称**: "Role of Glutaredoxin in Cardiac Ischemia-Reperfusion Injury: Insights from Antibody-Mediated Inhibition Studies"
**作者**: Chen L. et al.
**摘要**: 通过GLRX抗体阻断实验，研究发现心肌缺血再灌注损伤中GLRX通过调节硫醇氧化状态减轻细胞凋亡，为心脏保护提供了新的治疗靶点。
---
这些研究均利用GLRX抗体探索其在氧化应激、疾病机制及治疗中的功能，涵盖基础机制到临床应用的多个方向。
The glutaredoxin (GLRX) family comprises small, evolutionarily conserved redox enzymes critical for maintaining cellular redox homeostasis. These proteins, typically 10-12 kDa, belong to the thioredoxin superfamily and utilize a conserved cysteine-containing active site (CXXC motif) to catalyze thiol-disulfide exchange reactions. GLRX proteins primarily function in reducing glutathione-mixed disulfides, regenerating oxidized substrates, and regulating protein glutathionylation—a post-translational modification influencing protein activity and signaling pathways.
Three main isoforms (GLRX1. GLRX2. GLRX3) exist in mammals, with distinct subcellular localizations: GLRX1 is cytosolic, GLRX2 mitochondrial, and GLRX3 nuclear/cytosolic. Their roles span iron-sulfur cluster biosynthesis, antioxidant defense, and modulation of redox-sensitive transcription factors (e.g., NF-κB). Dysregulation of GLRX expression correlates with pathological conditions, including cancer progression, neurodegenerative disorders (e.g., Parkinson's), cardiovascular diseases, and diabetes, highlighting their involvement in oxidative stress responses.
GLRX antibodies are essential tools for detecting and quantifying these proteins in techniques like Western blotting, immunohistochemistry, and ELISA. They enable researchers to investigate GLRX expression patterns in disease models, assess subcellular distribution, and study interactions with client proteins. Commercial antibodies often target isoform-specific regions, aiding functional studies of individual GLRX members. Validated GLRX antibodies are crucial for advancing research into redox biology and developing therapeutic strategies targeting oxidative stress-related diseases.
×
