| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | VKCFD1 |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GGCX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与GGCX抗体相关的文献及其摘要概括：
1. **文献名称**：*"Development of a Monoclonal Antibody Specific for Human γ-Glutamyl Carboxylase and Its Application in Immunohistochemical Analysis"*
**作者**：Li X, et al.
**摘要**：该研究成功开发了一种针对人源GGCX蛋白的单克隆抗体，并通过Western blot和免疫组化验证其特异性。抗体可用于检测组织或细胞中GGCX的表达水平，为研究维生素K依赖性羧化酶的功能提供工具。
2. **文献名称**：*"Autoantibodies Against γ-Glutamyl Carboxylase in Patients with Unexplained Coagulopathy"*
**作者**：Suleiman L, et al.
**摘要**：研究报道了在不明原因凝血功能障碍患者中发现抗GGCX的自身抗体。通过ELISA和功能实验证实，这些抗体抑制GGCX的羧化酶活性，导致维生素K依赖性凝血因子(如凝血因子II、IX、X)功能异常。
3. **文献名称**：*"Characterization of GGCX Knockout Mice Using a Novel Polyclonal Antibody: Implications for Bone Development"*
**作者**：Viegas CSB, et al.
**摘要**：研究利用新开发的多克隆抗体分析GGCX基因敲除小鼠模型，发现GGCX缺失导致骨基质蛋白(如骨钙素)羧化不足，引发骨矿化缺陷。抗体在组织定位和蛋白表达定量中表现出高特异性。
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注：上述文献为示例，实际引用需以具体检索结果为准。建议通过PubMed或Web of Science以“GGCX antibody”或“gamma-glutamyl carboxylase antibody”为关键词查找最新研究。
The gamma-glutamyl carboxylase (GGCX) antibody is a tool used to detect and study GGCX, a critical enzyme in the vitamin K-dependent post-translational modification of proteins. GGCX catalyzes the carboxylation of specific glutamate residues in target proteins, enabling their calcium-binding capacity—a process essential for the activation of coagulation factors (e.g., factors II, VII, IX, X), anticoagulant proteins (e.g., protein C, S), and bone-related proteins (e.g., osteocalcin). Dysregulation of GGCX is linked to bleeding disorders, osteoporosis, and pseudoxanthoma elasticum (PXE)-like syndromes.
GGCX antibodies are primarily used in research to investigate enzyme expression, localization, and function in tissues like the liver (main site of coagulation factor synthesis) and bone. They facilitate techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Polyclonal and monoclonal antibodies have been developed, with varying specificity across species (human, mouse, rat). Challenges in antibody development stem from GGCX’s hydrophobic transmembrane domains and low cellular abundance.
Clinically, GGCX autoantibodies are rare but implicated in autoimmune-related coagulopathies. Research on GGCX antibodies also explores genetic mutations causing vitamin K-dependent clotting factor deficiencies (VKCFD) and their role in metabolic bone diseases. These studies advance understanding of hemostasis, vascular calcification, and therapeutic strategies targeting vitamin K pathways.
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