| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | EPF5; E2EPF; E2-EPF |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UBE2S |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2S抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit*
**作者**：Meyer, H.J., Rape, M.
**摘要**：该研究揭示UBE2S通过催化泛素链的延伸，调控APC/C复合体底物的降解，从而促进有丝分裂退出。研究中利用UBE2S抗体通过免疫印迹和免疫沉淀验证其与APC/C的相互作用及功能。
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2. **文献名称**：*Mechanisms of ubiquitin chain formation by the anaphase-promoting complex*
**作者**：Bremm, A., Komander, D.
**摘要**：文章阐明了UBE2S在APC/C介导的泛素化级联反应中的特异性作用，通过抗体阻断实验证明UBE2S对K11型泛素链形成的必要性，并解析其与底物结合的分子机制。
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3. **文献名称**：*Systematic analysis of human protein complexes identifies chromosome segregation proteins*
**作者**：Garnett, M.J., et al.
**摘要**：该大规模蛋白质组学研究通过UBE2S抗体进行免疫共沉淀筛选，发现UBE2S与染色体分离相关蛋白的关联，提示其在维持基因组稳定性中的潜在作用。
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*注*：以上文献为示例，实际引用时需核对真实存在的论文标题、作者及内容。建议通过PubMed或Google Scholar以“UBE2S antibody”、“UBE2S function”等关键词检索最新研究。
UBE2S (Ubiquitin-Conjugating Enzyme E2S) is a member of the ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS) responsible for targeted protein degradation. As an E2 enzyme, UBE2S facilitates the transfer of ubiquitin molecules to specific substrate proteins, typically in coordination with E3 ubiquitin ligases. This process, known as ubiquitination, tags proteins for proteasomal destruction or modulates their cellular localization and activity. UBE2S is particularly notable for its role in catalyzing the formation of K11-linked polyubiquitin chains, a specialized modification associated with cell cycle regulation and mitotic progression. Studies highlight its interaction with the anaphase-promoting complex/cyclosome (APC/C), where it contributes to the degradation of mitotic regulators, ensuring proper chromosome segregation and cell division.
In cancer research, UBE2S has garnered attention due to its frequent overexpression in various malignancies, including breast, liver, and colorectal cancers. Elevated UBE2S levels correlate with tumor aggressiveness, therapeutic resistance, and poor prognosis, suggesting its potential as a biomarker or therapeutic target. Antibodies targeting UBE2S are essential tools for investigating its expression patterns, subcellular localization, and functional mechanisms. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to dissect UBE2S's involvement in oncogenesis, DNA damage response, and cell cycle control. These antibodies also aid in exploring UBE2S's interplay with other UPS components and its regulatory pathways, advancing our understanding of protein homeostasis in health and disease.
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