| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DTNB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DTNB抗体的3篇示例参考文献(内容为模拟概括，仅供参考)：
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1. **文献名称**：*Development of a Monoclonal Antibody Specific for DTNB-Modified Proteins in Oxidative Stress Studies*
**作者**：Huang, Y. et al.
**摘要**：该研究开发了一种针对DTNB(5.5'-二硫代双(2-硝基苯甲酸)修饰蛋白的单克隆抗体，用于检测氧化应激条件下蛋白质巯基的氧化状态变化。抗体通过ELISA和Western blot验证，成功应用于细胞模型中氧化损伤标志物的检测。
2. **文献名称**：*DTNB-Based Antibody Probes for Quantifying Protein Thiol Redox Dynamics*
**作者**：Kwon, J. & Lee, S.
**摘要**：作者利用DTNB作为硫醇基团标记工具，结合定制抗体设计了一种新型探针，用于动态监测蛋白质巯基的氧化还原状态。该技术在小鼠糖尿病模型中验证，揭示了组织特异性氧化损伤机制。
3. **文献名称**：*Targeting DTNB-Conjugated Antigens: A Novel Approach in Neurodegenerative Disease Biomarker Discovery*
**作者**：Smith, R. et al.
**摘要**：研究通过DTNB修饰β-淀粉样蛋白制备抗原，并生成特异性抗体，用于阿尔茨海默病模型脑脊液样本中异常蛋白聚集物的检测，为神经退行性疾病诊断提供了潜在新工具。
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**注**：以上文献为示例，实际文献需通过PubMed、Web of Science等平台检索关键词如“DTNB antibody”“thiol labeling antibody”等获取。
The DTNB antibody is primarily associated with the detection of protein thiol groups modified by 5.5'-dithiobis-(2-nitrobenzoic acid) (DTNB), a widely used reagent in biochemistry for quantifying free sulfhydryl (-SH) groups. DTNB, also known as Ellman's reagent, reacts with thiols to form mixed disulfide bonds, releasing 2-nitro-5-thiobenzoic acid (TNB), which can be measured spectrophotometrically. Antibodies targeting DTNB-modified proteins are developed to specifically recognize TNB-conjugated residues, enabling the study of thiol redox states or protein conformational changes in biological systems.
These antibodies have applications in redox proteomics, where they help identify cysteine residues susceptible to oxidative modifications under stress conditions. They are also used in immunoassays (e.g., ELISA, Western blot) to detect oxidative damage or monitor protein structural alterations in diseases like neurodegenerative disorders, cardiovascular diseases, or cancer. Additionally, DTNB antibodies serve as tools to evaluate drug effects on cellular redox homeostasis or to validate thiol-blocking experimental conditions.
Their specificity for TNB-labeled epitopes makes them valuable in distinguishing oxidized versus reduced protein forms, aiding research on oxidative stress mechanisms and antioxidant defense systems. Proper controls are required to avoid cross-reactivity with other disulfide-bonded structures.
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