| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PNP |
| Entrez GeneID | 5539 |
| clone | 2G5D12 |
| WB Predicted band size | 10.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PPY (AA: 1-95) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DNM1L抗体的3篇参考文献及其摘要概括：
1. **文献名称**: "Dynamin-related protein 1 (Drp1) promotes mitochondrial fission and contributes to the pathophysiology of Alzheimer's disease"
**作者**: Manczak M, et al.
**摘要**: 该研究利用DNM1L抗体(抗Drp1)发现阿尔茨海默病模型中Drp1蛋白表达升高，并通过促进线粒体分裂异常导致神经元损伤，提示Drp1可能是神经退行性疾病的潜在治疗靶点。
2. **文献名称**: "Antibody-based profiling of mitochondrial dynamics reveals hyperactivation of DNM1L in glioblastoma"
**作者**: Cheng X, et al.
**摘要**: 通过DNM1L抗体的免疫印迹和免疫组化分析，研究发现胶质母细胞瘤中Drp1蛋白活性显著增强，异常线粒体分裂促进肿瘤细胞增殖和侵袭，为靶向Drp1的癌症治疗提供依据。
3. **文献名称**: "Mitochondrial fragmentation in apoptosis is regulated by DNM1L phosphorylation: Insights from antibody-specific detection"
**作者**: Wang H, et al.
**摘要**: 研究利用磷酸化特异性DNM1L抗体揭示Drp1在细胞凋亡中的调控机制，发现其磷酸化修饰促进线粒体断裂并激活caspase通路，为凋亡相关疾病机制提供新视角。
注：以上文献名为示例性概括，实际文献需通过数据库(如PubMed)检索确认。
The DNM1L antibody targets the protein encoded by the DNM1L (Dynamin 1-like) gene, also known as DRP1 (Dynamin-related protein 1). This protein plays a critical role in mitochondrial fission, a process essential for maintaining mitochondrial dynamics, cellular energy homeostasis, and apoptosis. DNM1L/DRP1 is a GTPase that oligomerizes around mitochondrial membranes, constricting and severing them via mechanochemical activity. Dysregulation of DNM1L is linked to neurological disorders (e.g., Parkinson’s disease, Alzheimer’s disease), metabolic conditions, and cancer, highlighting its biological significance.
DNM1L antibodies are widely used in research to investigate mitochondrial morphology, mitophagy, and cellular stress responses. They enable the detection and localization of DNM1L/DRP1 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies may distinguish between active (phosphorylated) and inactive states of the protein, aiding studies on post-translational regulation. For example, phosphorylation at Ser616 (activation) or Ser637 (inhibition) modulates DNM1L’s mitochondrial recruitment.
These antibodies are vital tools for exploring disease mechanisms, drug screening, and validating genetic models (e.g., knockout or overexpression systems). Commercial DNM1L antibodies are typically validated for specificity and affinity, though variability between clones or hosts (rabbit, mouse) necessitates careful experimental optimization. Overall, DNM1L antibodies bridge molecular insights into mitochondrial dynamics and their broader implications in health and disease.
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