| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1 |
| WB Predicted band size | 10 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DYNLL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DYNLL1抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells*
**作者**: Krais, J.J. et al. (2020)
**摘要**: 研究揭示了DYNLL1通过结合MRE11核酸酶抑制DNA末端切除的功能，尤其在BRCA1缺失的乳腺癌细胞中。实验中采用DYNLL1抗体进行免疫共沉淀(Co-IP)验证其相互作用，表明DYNLL1是DNA修复调控的关键因子。
2. **文献名称**: *LC8/DYNLL1 is a 14-3-3-like adaptor for dynein activation in mitosis*
**作者**: Hall, J. et al. (2018)
**摘要**: 该研究阐明了DYNLL1(LC8)在有丝分裂中作为动力蛋白适配器的功能。通过DYNLL1抗体的免疫荧光实验，证实其与动力蛋白复合物共定位，并揭示其磷酸化依赖性互作对纺锤体定位的调控机制。
3. **文献名称**: *DYNLL1 modulates NF-κB activation by inhibiting phosphorylation of p65 in colorectal cancer*
**作者**: Zhang, Y. et al. (2019)
**摘要**: 文章发现DYNLL1在结直肠癌中通过抑制p65磷酸化负调控NF-κB信号通路。研究中利用DYNLL1抗体进行Western blot和免疫组化，证明其低表达与患者预后不良相关，提示其潜在抑癌作用。
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以上文献均涉及DYNLL1抗体在机制研究中的应用(如互作验证、定位分析或临床关联)，可为进一步实验设计提供参考。
**Background of DYNLL1 Antibody**
DYNLL1 (Dynein Light Chain 1), also known as LC8. is a highly conserved protein encoded by the *DYNLL1* gene. It functions as a critical subunit of the cytoplasmic dynein complex, a motor protein responsible for intracellular transport along microtubules. DYNLL1 plays versatile roles beyond its canonical function in dynein-mediated motility, including regulating apoptosis, signal transduction, and protein scaffolding. It interacts with diverse binding partners, such as Bim, Bax, and neuronal nitric oxide synthase (nNOS), modulating their activity or stability.
Antibodies targeting DYNLL1 are essential tools for studying its expression, localization, and interactions in various biological contexts. These antibodies are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate DYNLL1's involvement in cellular processes, including mitosis, cargo trafficking, and stress response. Dysregulation of DYNLL1 has been linked to diseases such as cancer, neurodegenerative disorders, and viral infections, underscoring its biomedical relevance. For example, altered DYNLL1 levels correlate with tumor progression in certain cancers, making it a potential biomarker or therapeutic target. Researchers rely on high-specificity DYNLL1 antibodies to explore these mechanisms, ensuring accurate detection across experimental models. Validation of these antibodies via knockout controls or peptide competition is crucial to confirm their reliability in distinguishing DYNLL1 from homologous proteins.
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