| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | REDD2; Rtp801L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DDIT4L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DDIT4L抗体的模拟参考文献示例(请注意文献为虚构，仅作格式参考)：
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1. **文献名称**: "Characterization of a Novel Monoclonal Antibody for DDIT4L in Hypoxia Response"
**作者**: Zhang Q, et al.
**摘要**: 本研究开发了一种针对人DDIT4L蛋白的新型单克隆抗体，通过Western blot和免疫荧光验证其特异性。实验表明该抗体能有效检测缺氧条件下细胞中DDIT4L的上调表达，并揭示其与mTOR通路抑制的关联。
2. **文献名称**: "DDIT4L Expression Profiling in Renal Cell Carcinoma Using a Validated Polyclonal Antibody"
**作者**: Patel S, et al.
**摘要**: 文章报道了一种经过验证的多克隆抗体在肾癌组织中的应用，通过免疫组化证明DDIT4L在肿瘤低氧区域的高表达，并探讨其作为预后生物标志物的潜力，抗体特异性通过siRNA敲除实验确认。
3. **文献名称**: "Comparative Analysis of Commercial Anti-DDIT4L Antibodies for Flow Cytometry Applications"
**作者**: Müller F, et al.
**摘要**: 系统性比较了4种市售DDIT4L抗体的性能，发现Clone 5A3在流式细胞术中灵敏度最佳，适用于检测T细胞活化后DDIT4L的动态变化，同时指出不同抗体在固定/透化步骤中的表现差异。
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**提示**：实际文献需通过PubMed/Google Scholar检索关键词(如"DDIT4L antibody validation")。真实文献可能较少，建议结合抗体生产商的技术文档(如CST、Abcam等)作为补充。
The DNA Damage Inducible Transcript 4-Like (DDIT4L) protein, also known as REDD2 or RTP801L, is a stress-responsive regulator implicated in cellular processes such as apoptosis, autophagy, and metabolism. It belongs to the REDD1 protein family and shares structural homology with DDIT4/REDD1. DDIT4L is primarily induced under hypoxic, oxidative, or DNA-damaging conditions, where it acts as a key modulator of the mTOR (mammalian target of rapamycin) signaling pathway. By inhibiting mTORC1 activity, DDIT4L suppresses cell growth and promotes stress adaptation, linking cellular energy status to metabolic regulation. Dysregulation of DDIT4L has been associated with cancer, neurodegenerative disorders, and metabolic diseases, highlighting its therapeutic relevance.
DDIT4L antibodies are immunological tools developed to detect and quantify endogenous DDIT4L protein expression in research applications. These antibodies, often raised in rabbits or mice using recombinant DDIT4L antigens, enable the study of its expression patterns, subcellular localization, and interaction partners through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity validation via knockout controls is critical due to potential cross-reactivity with homologous proteins like DDIT4/REDD1. Researchers utilize DDIT4L antibodies to investigate its role in stress responses, tumor progression (where it may act as a tumor suppressor or promoter depending on context), and metabolic disorders. Recent studies also explore its involvement in aging-related processes and immune regulation, making these antibodies valuable for both basic research and preclinical investigations.
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