| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CHAC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于CHAC1抗体的3篇参考文献的简要总结：
---
1. **文献名称**：*CHAC1 is a cellular-specific mediator of ER stress signaling in antioxidative defense*
**作者**：Mungrue IN et al.
**摘要**：本研究揭示了CHAC1在调控内质网(ER)应激和氧化损伤中的作用，通过Western blot和免疫荧光实验验证了CHAC1抗体的特异性。发现CHAC1通过降解谷胱甘肽前体增强细胞凋亡，在动脉粥样硬化等疾病中起关键作用。
---
2. **文献名称**：*The role of CHAC1 in redox regulation and cisplatin resistance in ovarian cancer*
**作者**：Chen J et al.
**摘要**：作者利用CHAC1抗体检测卵巢癌细胞中CHAC1的表达水平，发现其与化疗耐药性相关。CHAC1通过调节氧化还原平衡影响癌细胞存活，抑制其表达可增强顺铂的敏感性。
---
3. **文献名称**：*CHAC1 degradation of glutathione accelerates oxidative stress and apoptosis in neurodegenerative models*
**作者**：Patel RK et al.
**摘要**：该研究通过敲低CHAC1并结合特异性抗体检测，证明其在神经退行性疾病中促进氧化应激和神经元凋亡。实验验证了CHAC1抗体在小鼠脑组织中的有效性，提示其作为治疗靶点的潜力。
---
以上文献均涉及CHAC1抗体的应用，涵盖疾病机制、药物反应及分子功能研究。
**Background of CHAC1 Antibody**
CHAC1 (Cation Transport Regulator-Like Protein 1) is an enzyme encoded by the *CHAC1* gene, belonging to the CHAC protein family. It plays a critical role in glutathione metabolism by catalyzing the degradation of glutathione, a key antioxidant, into 5-oxoproline and cysteinylglycine. This activity links CHAC1 to cellular redox regulation, apoptosis, and response to endoplasmic reticulum (ER) stress.
CHAC1 is induced under stress conditions, such as nutrient deprivation or unfolded protein response (UPR), and its overexpression promotes apoptosis by depleting intracellular glutathione levels, thereby increasing oxidative stress. Studies have implicated CHAC1 in various pathological processes, including cancer, neurodegenerative diseases, and cardiovascular disorders, where dysregulated glutathione metabolism contributes to disease progression.
The CHAC1 antibody is a crucial tool for detecting and quantifying CHAC1 protein expression in research. It enables the study of CHAC1's role in cellular stress responses, apoptosis pathways, and disease mechanisms through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Antibody specificity and validation are essential to ensure accurate detection, given the protein's low basal expression under normal conditions and its stress-induced upregulation.
Research utilizing CHAC1 antibodies has advanced understanding of its dual role as both a pro-apoptotic factor and a mediator of adaptive stress responses, highlighting its potential as a therapeutic target or biomarker in oxidative stress-related diseases.
×
