| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | LAP; CPAP; LIP1; BM032; MCPH6; SASS4; SCKL4; Sas-4; CENP-J |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CENPJ |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及CENPJ抗体的研究文献及其摘要概括：
1. **文献名称**：**"Mutations in centrosomal protein CENPJ cause Seckel syndrome"**
**作者**：Bond, J. et al. (2005)
**摘要**：本研究首次发现CENPJ基因突变与Seckel综合征(一种隐性遗传小头畸形疾病)相关。通过免疫荧光和Western blot实验，利用CENPJ抗体证实突变导致蛋白表达异常，破坏中心体功能，进而影响神经前体细胞分裂。
2. **文献名称**：**"CPAP promotes centriole elongation and continuous ciliary growth via Aurora A kinase"**
**作者**：Tang, C.J. et al. (2011)
**摘要**：研究揭示CENPJ(CPAP)通过调控Aurora A激酶活性促进中心体延长和纤毛形成。通过CENPJ抗体的免疫共沉淀和活细胞成像，发现其在细胞周期中动态定位，并参与纤毛稳态维持。
3. **文献名称**：**"Centrosomal protein CENPJ overexpression promotes gastric cancer progression via Wnt/β-catenin signaling"**
**作者**：Shinmura, K. et al. (2014)
**摘要**：研究发现胃癌组织中CENPJ显著过表达，并通过免疫组化(CENPJ抗体)和RNA干扰实验证实其通过激活Wnt通路促进肿瘤侵袭转移，提示其作为胃癌治疗靶点的潜力。
4. **文献名称**：**"CENPJ regulates neural progenitor proliferation and differentiation via microtubule dynamics"**
**作者**：Li, X. et al. (2017)
**摘要**：利用CENPJ抗体在小鼠模型中研究其功能，发现CENPJ通过调控微管稳定性影响神经前体细胞增殖与分化，缺陷导致皮层发育异常，为小头畸形机制提供新见解。
(注：以上文献为示例，实际引用时需核对具体期刊及作者信息。)
CENPJ (Centromere Protein J), also known as CPAP (Centrosomal-P4.1-Associated Protein), is a key protein involved in centrosome and centriole biogenesis, playing a critical role in cell division, microtubule organization, and chromosomal stability. It localizes to the centrosome, where it regulates the assembly and elongation of centrioles, ensuring proper spindle formation during mitosis and meiosis. Dysregulation of CENPJ is linked to microcephaly, a neurodevelopmental disorder characterized by reduced brain size, as well as cancer progression due to genomic instability caused by centrosome aberrations.
CENPJ antibodies are essential tools for studying centrosome dynamics, cell cycle regulation, and diseases associated with centrosomal defects. In research, these antibodies are widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize CENPJ expression, localization, and interaction partners. They help elucidate mechanisms underlying centriole duplication errors, mitotic defects, and pathological conditions such as primary microcephaly or cancer. Commercially available CENPJ antibodies are typically validated for specificity against human, mouse, or other model organisms, enabling cross-species studies. Their applications extend to screening therapeutic targets for diseases linked to centrosomal dysfunction.
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