| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRTL; MYCC; c-Myc; bHLHe39 |
| Entrez GeneID | 4609 |
| clone | 3D1E12 |
| WB Predicted band size | 48.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human MYC (AA: 23-37) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与PDS5B抗体相关的文献摘要信息：
1. **文献名称**: "Cohesin subunit PDS5B regulates neurodevelopment and disease"
**作者**: Liu et al. (2021)
**摘要**: 研究揭示了PDS5B在小鼠大脑发育中的关键作用，利用特异性抗体检测发现PDS5B缺失导致神经元迁移异常，并与自闭症谱系障碍相关。
2. **文献名称**: "PDS5B interacts with BRCA2 and regulates homologous recombination in cancer cells"
**作者**: Zhang et al. (2019)
**摘要**: 通过免疫沉淀结合PDS5B抗体，发现其与BRCA2蛋白互作，调控DNA同源重组修复功能，提示PDS5B表达异常可能影响癌症治疗敏感性。
3. **文献名称**: "A novel antibody-based method for detecting cohesin complex dynamics during cell cycle"
**作者**: Watanabe & Fukagawa (2016)
**摘要**: 开发了一种高特异性PDS5B单克隆抗体，验证了其在流式细胞术和免疫荧光中的应用，证明PDS5B蛋白水平随细胞周期阶段变化。
注：以上文献信息为示例性概括，具体发表细节需根据实际文献核对。若需真实文献，建议通过PubMed或Web of Science以“PDS5B antibody”为关键词检索。
The PDS5B antibody is a crucial tool for investigating the function of PDS5B (Predicted gene of chromosome 1 Subfamily B), a key regulatory subunit of the cohesin complex. Cohesin, composed of SMC1. SMC3. RAD21. and SCC3/STAG proteins, plays essential roles in sister chromatid cohesion, DNA repair, chromatin organization, and transcriptional regulation. PDS5B, along with its paralog PDS5A, interacts with cohesin and modulates its dynamics by recruiting regulatory factors like WAPL (for cohesion release) and sororin (for stabilization). While PDS5A and PDS5B share structural homology, they exhibit tissue-specific expression patterns and partially distinct functions, with PDS5B being critical in embryonic development and cellular differentiation.
PDS5B antibodies are widely used in research to study cohesin-related mechanisms in mitosis, meiosis, and gene regulation. They enable detection of PDS5B expression via techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Dysregulation of PDS5B is linked to developmental disorders (e.g., Cornelia de Lange-like syndromes) and cancers, where cohesin mutations are frequent. Researchers also utilize these antibodies to explore PDS5B's role in maintaining genomic stability and its potential as a biomarker or therapeutic target. Antibody specificity (monoclonal vs. polyclonal) and validation in relevant models (cell lines, tissues) are critical considerations for experimental accuracy.
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