| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GCN2; PVOD2 |
| WB Predicted band size | 187 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EIF2AK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于 **EIF2AK4(GCN2)抗体** 的模拟参考文献示例(内容为虚构，建议通过学术数据库验证真实文献)：
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1. **文献名称**: *EIF2AK4 mutations in pulmonary arterial hypertension: a genetic and functional study*
**作者**: Eyries, M., et al.
**摘要**: 本研究通过全外显子测序发现EIF2AK4基因突变与遗传性肺动脉高压(HPAH)相关，利用特异性抗体检测患者组织中EIF2AK4蛋白表达缺失，揭示其通过调控eIF2α磷酸化影响血管平滑肌细胞凋亡的机制。
2. **文献名称**: *GCN2 kinase inhibition sensitizes cancer cells to amino acid deprivation stress*
**作者**: Chantranupong, L., et al.
**摘要**: 文章开发了一种抗EIF2AK4(GCN2)的单克隆抗体，用于检测肿瘤微环境中GCN2的激活状态，证明其通过调控mTORC1通路促进肿瘤细胞在氨基酸缺乏条件下的存活。
3. **文献名称**: *EIF2AK4 antibody-based detection of unfolded protein response in autoimmune disorders*
**作者**: Smith, J., & Jones, R.
**摘要**: 通过Western blot和免疫组化分析，研究利用EIF2AK4抗体证明类风湿关节炎患者滑膜组织中内质网应激反应增强，提示GCN2信号通路在自身免疫炎症中的潜在作用。
4. **文献名称**: *Development and validation of a high-specificity EIF2AK4 polyclonal antibody for translational research*
**作者**: Brown, K., et al.
**摘要**: 报道了一种新型兔源多克隆抗体的制备方法，该抗体针对EIF2AK4的C端结构域，验证了其在ELISA、免疫荧光及IP实验中的高特异性，为相关疾病模型研究提供工具。
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**注意**：以上文献为示例，实际研究请通过PubMed、Google Scholar等平台检索关键词“EIF2AK4 antibody”或“GCN2 antibody”获取真实文献。
The EIF2AK4 antibody is a tool used to detect eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), also known as GCN2. a serine/threonine kinase critical in cellular stress responses. EIF2AK4 regulates the integrated stress response (ISR) by phosphorylating the alpha subunit of eukaryotic initiation factor 2 (eIF2α) under conditions such as amino acid deprivation, UV exposure, or viral infection. This phosphorylation halts global protein synthesis while promoting the translation of stress-responsive genes, enabling cells to adapt to adverse conditions.
Research on EIF2AK4 has gained attention due to its role in diseases like pulmonary arterial hypertension (PAH). Biallelic mutations in the EIF2AK4 gene are linked to heritable pulmonary veno-occlusive disease/pulmonary capillary hemangiomatosis (PVOD/PCH), a rare and severe form of PAH. Antibodies targeting EIF2AK4 are essential for studying its expression, activation, and interaction networks in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore its tissue distribution, signaling pathways, and potential therapeutic targeting.
Understanding EIF2AK4’s function through antibody-based assays may unveil novel insights into stress adaptation mechanisms and inform treatments for conditions involving dysregulated ISR, including cancer, neurodegenerative disorders, and metabolic diseases.
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