| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PFKF; PFK-C |
| WB Predicted band size | 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PFKP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PFKP抗体的参考文献(信息基于公开文献概括，非真实存在，仅作示例)：
1. **《PFKP调控胶质瘤糖代谢重编程的机制研究》**(作者：Zhang et al., 2020)
摘要：研究使用PFKP特异性抗体通过Western blot和免疫组化分析，发现PFKP在胶质瘤中高表达，并通过激活糖酵解途径促进肿瘤细胞增殖，提示其作为治疗靶点的潜力。
2. **《PFKP在肺癌转移中的功能及临床意义》**(作者：Li et al., 2018)
摘要：通过PFKP抗体检测肺癌患者组织样本，证实PFKP表达与转移正相关，机制研究表明其通过调控HIF-1α通路增强肿瘤侵袭性，为预后标志物开发提供依据。
3. **《靶向PFKP的抗体在乳腺癌代谢成像中的应用》**(作者：Wang et al., 2021)
摘要：开发了高特异性PFKP单克隆抗体，结合免疫荧光和流式细胞术，揭示PFKP在乳腺癌细胞线粒体-胞浆间的动态分布，为代谢成像技术提供新工具。
(注：以上为模拟文献，实际文献需通过PubMed/Google Scholar以“PFKP antibody”“PFKP cancer”等关键词检索。)
The PFKP antibody targets phosphofructokinase-platelet type (PFKP), a key regulatory enzyme in glycolysis. PFKP catalyzes the phosphorylation of fructose-6-phosphate to fructose-1.6-bisphosphate, a rate-limiting step that controls glycolytic flux. As one of three PFK isoforms (PFKM in muscle, PFKL in liver, PFKP in platelets), it is predominantly expressed in platelets, brain, and placenta but also upregulated in various cancers. Dysregulation of PFKP is linked to metabolic reprogramming in tumors, supporting the Warburg effect by enhancing aerobic glycolysis for rapid energy production.
PFKP antibodies are essential tools for studying its expression, localization, and functional roles in physiological and pathological contexts. They enable detection via Western blotting, immunohistochemistry, and immunofluorescence, aiding research on cancer metabolism, neurodegenerative disorders (e.g., Alzheimer’s), and platelet-related diseases. Recent studies also explore PFKP’s non-metabolic roles in regulating autophagy, cell proliferation, and inflammation.
Commercial PFKP antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown. Researchers prioritize antibodies with high affinity and minimal cross-reactivity against PFKL/PFKM isoforms. Understanding PFKP dynamics offers therapeutic insights, as targeting glycolytic enzymes may improve strategies against cancer and metabolic syndromes.
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