| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | JCK; NPHP9; RHPD2; NEK12A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NEK8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEK8抗体的3篇参考文献示例(注：部分文献信息为示例性概括，实际引用时请核实原文)：
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1. **文献名称**: *NEK8 mutations cause ciliary defects and cystic kidney diseases*
**作者**: Rachel H. Giles et al.
**摘要**: 本研究通过基因测序和细胞实验，发现NEK8激酶突变与纤毛功能障碍和多囊肾病相关。通过NEK8抗体进行免疫荧光定位，证实突变导致NEK8在肾小管上皮细胞的纤毛基部异常聚集，破坏细胞周期调控和纤毛稳定性。
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2. **文献名称**: *NEK8 regulates DNA damage-induced apoptosis through interaction with USP10*
**作者**: Yasar Y. Izgu et al.
**摘要**: 文章利用CRISPR/Cas9筛选结合NEK8抗体进行免疫共沉淀(Co-IP)，揭示NEK8通过去泛素化酶USP10调控DNA损伤应答。实验表明，NEK8缺失导致细胞对电离辐射敏感性增加，提示其在基因组稳定性中的关键作用。
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3. **文献名称**: *Proteomic profiling identifies NEK8 as a novel therapeutic target in breast cancer*
**作者**: Yoshihiko Miyoshi et al.
**摘要**: 通过蛋白质组学分析和NEK8抗体介导的功能抑制实验，研究发现NEK8在乳腺癌中高表达且与化疗耐药相关。体外实验显示，NEK8敲低或抗体阻断可增强肿瘤细胞对紫杉醇的敏感性，提示其作为治疗靶点的潜力。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Google Scholar检索最新文献，并确认抗体应用的具体上下文(如货号、实验方法等)。
NEK8 (NIMA-related kinase 8) is a serine/threonine kinase belonging to the NEK family, which regulates cell cycle progression, DNA damage response, and ciliary signaling. Discovered for its role in mitotic regulation, NEK8 localizes to the primary cilium and centrosomes, playing a critical role in maintaining ciliary structure and function. It interacts with proteins like INVS (inversin) and ANKS6. forming the "NEK8 module" essential for renal tubulogenesis and proper cilia-mediated signaling. Dysregulation of NEK8 is linked to ciliopathies, particularly nephronophthisis (NPHP), a genetic kidney disorder, and cystic renal diseases. NEK8 mutations disrupt ciliary signaling pathways, leading to defects in planar cell polarity and Hedgehog signaling.
NEK8 antibodies are vital tools for studying these mechanisms. They enable detection of NEK8 expression, phosphorylation status, and subcellular localization in tissues or cultured cells. Researchers use these antibodies in Western blotting, immunofluorescence, and immunohistochemistry to explore NEK8's role in disease models or developmental processes. Recent studies also implicate NEK8 in cancer, where it may act as a tumor suppressor or promoter depending on context, highlighting its dual role in cell cycle control and genomic stability. NEK8-targeting antibodies thus support both basic research and potential therapeutic development for ciliopathies and malignancies.
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