| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NLK1; NEK2A; HsPK21 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NEK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEK2抗体的参考文献示例(注：以下为模拟内容，实际文献请通过学术数据库查询)：
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1. **文献名称**：NEK2 promotes tumor cell proliferation and correlates with poor prognosis in breast cancer
**作者**：Smith A, et al.
**摘要**：该研究通过免疫组化(使用NEK2特异性抗体)分析了乳腺癌组织中NEK2的表达水平，发现NEK2高表达与肿瘤增殖加速及患者生存率下降显著相关，提示其作为乳腺癌预后标志物的潜力。
2. **文献名称**：Role of NEK2 in colorectal cancer metastasis: Insights from antibody-mediated knockdown
**作者**：Chen L, et al.
**摘要**：研究利用NEK2抗体进行Western blot和免疫荧光实验，证实NEK2在结直肠癌转移中通过调控EMT(上皮间质转化)通路发挥作用，靶向NEK2可抑制癌细胞侵袭能力。
3. **文献名称**：NEK2 kinase as a therapeutic target in hepatocellular carcinoma
**作者**：Wang X, et al.
**摘要**：通过NEK2抗体检测肝癌临床样本，发现NEK2表达与肿瘤分级正相关。体内实验显示，抑制NEK2活性可显著减少肿瘤生长，支持其作为肝细胞癌治疗靶点的可行性。
4. **文献名称**：NEK2 regulates mitotic spindle assembly in human somatic cells
**作者**：Johnson R, et al.
**摘要**：该研究利用NEK2抗体进行免疫沉淀和共聚焦显微镜分析，揭示了NEK2在有丝分裂纺锤体组装中的关键作用，并发现其缺失导致染色体不稳定，与癌症发生相关。
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如需具体文献，建议访问PubMed、Web of Science或Google Scholar，以关键词“NEK2 antibody”、“NEK2 cancer”等检索最新研究。
NEK2 (NIMA-related kinase 2) is a serine/threonine kinase belonging to the NEK family, which plays a critical role in regulating cell cycle progression, particularly during the G2/M phase transition and mitosis. It is involved in centrosome maturation, spindle assembly, and chromosome alignment, ensuring genomic stability. Dysregulation of NEK2 is linked to various cancers, where its overexpression correlates with tumor progression, chemotherapy resistance, and poor prognosis. NEK2 activity is tightly controlled through phosphorylation and ubiquitination, with its protein levels peaking during mitosis before degradation via the ubiquitin-proteasome system.
NEK2 antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Due to the presence of NEK2 isoforms (e.g., NEK2A and NEK2B), antibodies are often designed to target specific regions, such as the kinase domain or unique C-terminal sequences, to ensure isoform specificity. Some antibodies also detect phosphorylation states (e.g., Thr175), aiding in the study of NEK2 activation mechanisms.
Commercially available NEK2 antibodies support research into its oncogenic roles and potential as a therapeutic target. However, users must validate antibody specificity for their experimental models, as cross-reactivity with other NEK family members or isoforms can occur. Ongoing studies explore NEK2 inhibitors for cancer treatment, underscoring the antibody's value in both basic research and drug development.
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