| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NEK11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEK11抗体的3篇模拟参考文献及其摘要概括：
1. **文献名称**: *Development of a Polyclonal Antibody Against Human NEK11 for Detection of Endogenous Protein Expression*
**作者**: Smith A, et al.
**摘要**: 该研究报道了一种针对人NEK11激酶的多克隆抗体的开发与验证。通过免疫印迹和免疫荧光实验，证实该抗体能特异性识别内源性NEK11蛋白，并适用于多种细胞系的检测，为后续功能研究提供了工具。
2. **文献名称**: *NEK11-Mediated Phosphorylation of p53 in DNA Damage Response*
**作者**: Tanaka K, et al.
**摘要**: 本文利用NEK11特异性抗体探究了NEK11在DNA损伤应答中的作用。研究发现，NEK11通过磷酸化p53蛋白调控细胞周期停滞，抗体在免疫沉淀和染色质结合实验中验证了NEK11与DNA修复通路的关联。
3. **文献名称**: *NEK11 Expression as a Prognostic Marker in Colorectal Cancer: An Immunohistochemical Study*
**作者**: Chen L, et al.
**摘要**: 研究使用NEK11抗体对结直肠癌组织进行免疫组化分析，发现NEK11的高表达与患者不良预后显著相关。该抗体在临床样本中的稳定表现支持其作为潜在生物标志物的应用。
**注**：以上文献为模拟示例，实际研究中需查阅真实数据库获取具体信息。
The NEK11 (NIMA-related kinase 11) antibody is a tool used to study the NEK11 protein, a member of the NIMA-related kinase family involved in cell cycle regulation, DNA damage response, and checkpoint control. NEK11 plays a critical role in the G2/M phase transition, particularly in response to replication stress, by interacting with key regulators like ATR, CHK1. and WEE1. It contributes to maintaining genomic stability through phosphorylation events that delay cell cycle progression, allowing time for DNA repair. NEK11 exists in two major splice variants (NEK11L and NEK11S) with distinct regulatory roles.
Research using NEK11 antibodies has linked its dysregulation to cancer progression, including breast, colorectal, and lung cancers, where overexpression often correlates with poor prognosis. The antibody is essential for detecting NEK11 expression levels, phosphorylation status (e.g., at Ser273/275), and subcellular localization in experimental models. It is widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore NEK11’s functional mechanisms or therapeutic potential.
Despite its established roles, NEK11’s full biological significance remains under investigation, with some studies highlighting context-dependent interactions and conflicting data on its pro- or anti-tumor effects. The antibody’s specificity and validation across species (human, mouse, rat) are critical for reliable research outcomes.
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