| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PI; A1A; AAT; PI1; A1AT; SERPINA1 |
| Entrez GeneID | 5265 |
| clone | 2B12 |
| WB Predicted band size | 47kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human AAT expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于PAPSS1抗体的参考文献示例(注：文献为示例性概括，非真实存在)：
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1. **文献名称**: *PAPSS1 regulates sulfation pathways in chondrogenesis via antibody-based localization analysis*
**作者**: Smith A, et al.
**摘要**: 本研究利用兔源多克隆PAPSS1抗体，通过免疫组化技术揭示了PAPSS1在小鼠软骨细胞中的亚细胞定位，证明其在硫酸化代谢中的关键作用，并发现其表达缺失导致骨骼发育异常。
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2. **文献名称**: *Development and validation of a monoclonal antibody for specific detection of PAPSS1 in cancer models*
**作者**: Chen L, et al.
**摘要**: 作者开发了一种高特异性小鼠单克隆PAPSS1抗体，通过Western blot和流式细胞术验证其在乳腺癌细胞系中的灵敏度，证实PAPSS1低表达与肿瘤转移相关，为癌症诊断提供新工具。
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3. **文献名称**: *PAPSS1 knockout mice exhibit metabolic disorders revealed by antibody-mediated protein profiling*
**作者**: Tanaka K, et al.
**摘要**: 研究通过构建PAPSS1基因敲除小鼠，使用商业化山羊源PAPSS1抗体进行组织蛋白印迹分析，发现PAPSS1缺失导致肝脏硫酸盐代谢紊乱，并引发脂质异常积累。
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(若需真实文献，建议通过PubMed或Google Scholar以“PAPSS1 antibody”+“Western blot/immunohistochemistry”等关键词检索，并筛选涉及抗体应用的研究。)
**Background of PAPSS1 Antibody**
The PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate synthase 1) antibody is a tool used to detect and study the PAPSS1 enzyme, a key player in cellular sulfation pathways. PAPSS1 catalyzes the synthesis of 3'-phosphoadenosine-5'-phosphosulfate (PAPS), the universal sulfate donor for sulfotransferase-mediated reactions. These reactions are critical for sulfating diverse substrates, including proteoglycans, hormones, neurotransmitters, and xenobiotics, influencing their stability, activity, and clearance.
PAPSS1 localizes primarily to the cytosol and nucleus, with tissue-specific expression in the liver, cartilage, and adrenal glands. Dysregulation of PAPSS1 is linked to skeletal disorders (e.g., brachyolmia), hormonal imbalances, and cancer progression. For instance, reduced PAPSS1 expression may impair sulfation of extracellular matrix components, contributing to tumor metastasis or cartilage defects.
The PAPSS1 antibody enables researchers to investigate protein expression, subcellular localization, and molecular interactions in various models. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies leveraging this antibody have clarified PAPSS1's role in metabolic regulation, developmental biology, and disease mechanisms. Its specificity and reliability make it essential for exploring PAPSS1's contributions to sulfation-dependent processes and potential therapeutic targets.
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