| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CART1; MLN62; RNF83 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TRAF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRAF4抗体的3篇代表性文献(示例格式，内容为模拟整合)：
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1. **文献名称**: "TRAF4 is a critical regulator of breast cancer progression through NF-κB signaling"
**作者**: Li Y, et al.
**摘要**: 研究利用TRAF4抗体进行免疫沉淀和Western blot分析，发现TRAF4通过激活NF-κB通路促进乳腺癌细胞增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**: "TRAF4 modulates TGF-β signaling in lung adenocarcinoma"
**作者**: Wang H, et al.
**摘要**: 通过免疫组化(使用TRAF4特异性抗体)和基因沉默实验，揭示TRAF4在肺腺癌中调控TGF-β/Smad信号通路的机制，并与患者预后不良相关。
3. **文献名称**: "Antibody-based profiling of TRAF4 expression in colorectal cancer"
**作者**: Zhang R, et al.
**摘要**: 研究开发了一种高特异性TRAF4单克隆抗体，用于结直肠癌组织芯片分析，证实TRAF4高表达与肿瘤分期和淋巴结转移显著相关。
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*注：以上为示例性内容，实际文献需通过PubMed或Google Scholar检索关键词“TRAF4 antibody”或“TRAF4 function”获取。如需具体文献，建议提供更详细的研究方向或应用场景。*
TRAF4 (Tumor Necrosis Factor Receptor-Associated Factor 4) is an adaptor protein belonging to the TRAF family, which plays critical roles in regulating immune responses, cellular stress pathways, and developmental processes. Unlike other TRAF members (e.g., TRAF2. TRAF6), TRAF4 is unique in its structural features, including a conserved TRAF domain, a zinc-binding RING finger motif, and a coiled-coil region. It interacts with diverse signaling pathways, such as NF-κB, MAPK, and Wnt, and is implicated in cell survival, polarity, and migration.
TRAF4 is overexpressed in various cancers (e.g., breast, lung, prostate) and linked to tumor progression, metastasis, and therapy resistance. It also participates in neurological disorders and developmental defects. TRAF4 antibodies are essential tools for detecting TRAF4 expression and studying its function. These antibodies are typically generated against specific epitopes, such as the N-terminal or TRAF domain, and validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Commercial clones (e.g., D4G6 from Cell Signaling Technology) are widely used in research to explore TRAF4's role in disease mechanisms or its interaction networks. Selecting a TRAF4 antibody requires attention to specificity, host species, and validation data to avoid cross-reactivity with other TRAF proteins. Its study holds potential for therapeutic targeting in oncology and beyond.
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