| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21 |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TFF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TFF1抗体的3篇参考文献及其简要摘要：
1. **"TFF1 deficiency induces epithelial-mesenchymal transition and enhances sensitivity to gastric carcinogens"**
- **作者**: Machado JC, et al.
- **摘要**: 该研究通过免疫组化(使用TFF1抗体)发现，TFF1在胃癌中常表达缺失，其缺失与黏膜屏障功能受损相关，可能促进肿瘤侵袭和转移。
2. **"Trefoil factor 1 suppression of innate immune responses to Helicobacter pylori infection"**
- **作者**: Soutto M, et al.
- **摘要**: 文章利用TFF1抗体检测幽门螺杆菌感染模型中的蛋白表达，发现TFF1通过调节NF-κB通路抑制炎症反应，保护胃黏膜但可能促进慢性感染。
3. **"DNA methylation of TFF1 in gastric epithelial differentiation and carcinogenesis"**
- **作者**: Bessaoud F, et al.
- **摘要**: 研究采用TFF1抗体分析胃癌组织，发现TFF1启动子甲基化导致其表达下调，与分化异常和早期癌变相关，提示其作为潜在诊断标志物。
4. **"TFF1 modulates NF-κB signaling and cytokine production in intestinal inflammation"**
- **作者**: Thoma SB, et al.
- **摘要**: 通过Western blot(使用TFF1抗体)揭示TFF1在结肠炎中调控炎症因子分泌，其缺失加剧肠道损伤，可能成为炎症性肠病治疗靶点。
以上文献均涉及TFF1抗体在疾病机制研究中的应用，涵盖癌症、感染和炎症领域。
**Background of TFF1 Antibody**
TFF1 (Trefoil Factor Family 1), also known as pS2 or breast cancer estrogen-inducible protein, is a small secretory protein belonging to the trefoil factor family. It is characterized by a conserved trefoil domain containing three disulfide bonds, which stabilizes its structure in harsh environments like the gastrointestinal lumen. TFF1 is primarily expressed in mucosal epithelial cells of the stomach, colon, and mammary glands, where it plays a critical role in mucosal protection, repair, and restitution through anti-apoptotic, mitogenic, and migratory activities.
TFF1 has dual roles in cancer, acting as a tumor suppressor or promoter depending on context. Loss of TFF1 expression is linked to gastric carcinogenesis, while its overexpression in estrogen receptor-positive (ER+) breast cancer correlates with poor prognosis. TFF1 antibodies are essential tools for studying these mechanisms. They enable detection of TFF1 expression in tissues (via immunohistochemistry) or bodily fluids (via ELISA/Western blot), aiding in cancer diagnosis, prognosis, and research into therapeutic targeting.
Recent studies also explore TFF1's involvement in inflammation, metabolic disorders, and its potential as a biomarker. However, inconsistent findings across studies highlight the need for standardized antibody validation to ensure specificity and reproducibility in experimental and clinical applications.
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