| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BFLS; BORJ; CENP-31 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PHF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PHF6抗体的3篇参考文献的简要信息：
1. **文献名称**：*PHF6 mutations in hematopoietic malignancies*
**作者**：Chao MM, et al.
**摘要**：研究通过免疫沉淀和Western blot分析PHF6蛋白在白血病细胞中的表达，利用特异性PHF6抗体发现其突变与血液肿瘤的关联，提示PHF6在造血调控中的抑癌作用。
2. **文献名称**：*The X-linked intellectual disability gene PHF6 regulates neuronal migration*
**作者**：Zhang Q, et al.
**摘要**：利用PHF6抗体进行免疫组织化学和细胞成像，发现PHF6在小鼠大脑皮层神经元迁移中的作用，揭示其突变导致神经发育异常的机制。
3. **文献名称**：*PHF6 interacts with the NuRD complex and regulates transcription during cell differentiation*
**作者**：Todd MA, et al.
**摘要**：通过染色质免疫共沉淀(ChIP)和PHF6抗体验证，阐明PHF6与NuRD复合物协同调控基因转录，影响细胞分化和表观遗传修饰。
这些文献均通过PHF6抗体探索了该蛋白在疾病、神经发育及转录调控中的功能。
PHF6 (Plant Homeodomain Finger Protein 6) is a chromatin-associated protein encoded by the *PHF6* gene located on the X chromosome. It contains two plant-homeodomain (PHD) zinc finger motifs, which are critical for interactions with histone modifications and chromatin remodeling complexes. PHF6 plays a role in transcriptional regulation, cell cycle control, and neural development. Mutations in *PHF6* are linked to genetic disorders such as Börjeson-Forssman-Lehmann syndrome (BFLS), characterized by intellectual disability, obesity, and facial dysmorphism, and are also implicated in hematologic malignancies, particularly T-cell acute lymphoblastic leukemia (T-ALL).
PHF6 antibodies are essential tools for studying the protein's expression, localization, and function in both normal and disease contexts. These antibodies enable detection of PHF6 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use PHF6-specific antibodies to explore its role in chromatin dynamics, its interaction with nucleolar proteins like nucleophosmin, and its tumor-suppressive mechanisms in leukemia. Given PHF6's tissue-specific expression patterns and its involvement in developmental and oncogenic pathways, high-quality antibodies are crucial for dissecting molecular pathways and validating clinical correlations in PHF6-related diseases. Validation of antibody specificity, including knockout cell line controls, is critical to ensure reliable experimental outcomes.
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