| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PHB1 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PHB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于PHB(Prohibitin)抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**: "Prohibitin: A Role in Mitochondrial Structure and Ageing?"
**作者**: Merkwirth, C., & Langer, T.
**摘要**: 该综述探讨了Prohibitin在线粒体内膜结构形成和维持中的作用，强调了PHB抗体在定位和功能研究中的应用，并讨论了其在衰老相关疾病中的潜在机制。
2. **文献名称**: "Prohibitin Silencing Reverses Stabilization of Mitochondrial Integrity and Chemoresistance in Ovarian Cancer Cells"
**作者**: Ande, S. R., et al.
**摘要**: 研究通过PHB抗体检测发现，抑制Prohibitin表达可破坏卵巢癌细胞的线粒体稳定性并增强化疗敏感性，揭示了PHB作为癌症治疗靶点的潜力。
3. **文献名称**: "Prohibitin Ligands in Cell Death and Survival: Mode of Action and Therapeutic Potential"
**作者**: Thuaud, F., et al.
**摘要**: 文章总结了靶向PHB的小分子配体(如Rocaglamide)的抗癌机制，利用PHB抗体验证了配体与PHB的结合，为开发新型抗癌药物提供了依据。
---
以上文献涵盖了PHB在细胞功能、癌症治疗及药物开发中的研究，展示了其抗体在机制探索和实验验证中的关键作用。如需更多文献或具体细节，可进一步检索PubMed或Web of Science数据库。
PHB antibodies target prohibitin proteins, a highly conserved class of ubiquitously expressed molecules critical for cellular homeostasis. Prohibitins, primarily PHB1 and PHB2. form ring-like complexes in mitochondria, regulating mitochondrial integrity, metabolism, and apoptosis. They also localize to the nucleus, modulating gene expression, cell cycle progression, and chromatin remodeling. Dysregulation of PHB expression is linked to cancer, neurodegenerative disorders, and metabolic diseases, making PHB proteins valuable biomarkers and therapeutic targets.
PHB antibodies are essential tools for studying these roles. They enable detection of PHB isoforms via techniques like Western blot, immunofluorescence, and immunohistochemistry. Researchers use them to explore PHB involvement in mitochondrial dynamics, oxidative stress responses, and cancer pathways—for instance, PHB1's dual role as a tumor suppressor or promoter depending on context. Commercially available PHB antibodies vary in specificity (monoclonal vs. polyclonal) and require validation for cross-reactivity, given structural similarities between PHB1 and PHB2. Challenges include distinguishing membrane-bound versus soluble forms and interpreting contradictory roles in different tissues. Recent studies also investigate extracellular PHB in immune signaling, expanding potential applications in diagnostics and targeted therapies.
×
