| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MEK5, MAPKK5, PRKMK5, HsT17454 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAP2K5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAP2K5抗体的简要参考文献示例(内容为虚构，仅供参考)：
1. **文献名称**：*MAP2K5 Antibody Validation in Cardiac Hypertrophy Models*
**作者**：Li, X., et al.
**摘要**：该研究通过Western blot和免疫组化验证了MAP2K5抗体在小鼠心脏肥大模型中的特异性，发现MAP2K5表达上调与心肌细胞增殖相关。
2. **文献名称**：*Development of a Novel Monoclonal Antibody for MAP2K5 and Its Application in Cancer Research*
**作者**：Zhang, Y., et al.
**摘要**：研究者开发了一种高特异性MAP2K5单克隆抗体，并用于检测多种癌症组织中MAP2K5的异常表达，提示其与肿瘤侵袭性的潜在关联。
3. **文献名称**：*MAP2K5 Signaling in Neurodegenerative Diseases: Insights from Antibody-Based Detection*
**作者**：Smith, J., et al.
**摘要**：利用MAP2K5抗体分析阿尔茨海默病模型脑组织，发现MAP2K5信号通路激活与tau蛋白过度磷酸化存在相关性。
4. **文献名称**：*A Comparative Study of Commercial MAP2K5 Antibodies for Flow Cytometry Applications*
**作者**：Wang, H., et al.
**摘要**：对比了多种市售MAP2K5抗体的灵敏度和特异性，为流式细胞术中MAP2K5蛋白检测提供了优化方案。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed或Web of Science。)
The MAP2K5 antibody is a valuable tool for studying the mitogen-activated protein kinase kinase 5 (MAP2K5 or MEK5), a critical component of the MAPK/ERK signaling pathway. MAP2K5 functions as a dual-specificity kinase that phosphorylates and activates its downstream target, MAPK7 (ERK5), in response to extracellular stimuli such as stress, growth factors, or cytokines. This pathway regulates diverse cellular processes, including proliferation, differentiation, survival, and apoptosis. Structurally, MAP2K5 contains an N-terminal catalytic domain and a C-terminal regulatory region, with phosphorylation at specific residues (e.g., Ser311/Thr315) essential for its activation.
Antibodies targeting MAP2K5 are widely used in Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels, phosphorylation status, and subcellular localization in various tissues or cell lines. These antibodies help elucidate MAP2K5's role in physiological processes like angiogenesis and tissue development, as well as pathological conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders. Dysregulation of the MAP2K5-ERK5 axis is linked to tumor progression, metastasis, and therapy resistance, making it a potential therapeutic target. Researchers also utilize MAP2K5 antibodies to assess signaling pathway activity in drug discovery and mechanistic studies. Validation via knockout/knockdown controls ensures specificity, enabling accurate interpretation of experimental results.
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