| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CR6, DDIT2, GRP17, GADD45gamma |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GADD45G |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GADD45G抗体的3篇参考文献示例(文献信息及摘要内容为模拟生成，仅供参考)：
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1. **文献名称**: "GADD45G regulates cell cycle arrest and DNA repair in response to UV irradiation"
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性GADD45G抗体，通过Western blot和免疫荧光技术，验证了GADD45G在紫外线诱导的DNA损伤后细胞周期阻滞中的作用，并发现其与PCNA蛋白的相互作用促进DNA修复。
2. **文献名称**: "Development of a monoclonal antibody against human GADD45G for cancer biomarker analysis"
**作者**: Li X, et al.
**摘要**: 研究团队开发了一种高特异性抗人GADD45G单克隆抗体，通过ELISA和免疫组化验证其在结直肠癌组织中的表达水平，表明GADD45G低表达与患者预后不良相关。
3. **文献名称**: "GADD45G modulates oxidative stress-induced apoptosis in neuronal cells"
**作者**: Park S, et al.
**摘要**: 使用GADD45G抗体在小鼠神经元细胞中检测到，GADD45G通过激活p38 MAPK通路调控氧化应激下的细胞凋亡，提示其在神经退行性疾病中的潜在作用。
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*注：以上文献为示例，实际引用需查询PubMed或Web of Science等数据库获取真实文献。若需具体文章，建议检索关键词 "GADD45G antibody" 或 "GADD45G immunohistochemistry/Western blot"。*
The GADD45G (Growth Arrest and DNA Damage-Inducible 45 Gamma) antibody is a key tool for studying the GADD45G protein, a member of the evolutionarily conserved GADD45 family involved in cellular stress responses. GADD45G is induced by various stressors, including DNA damage, oxidative stress, and cytokine signaling. It regulates critical processes such as cell cycle arrest (primarily at G1/S and G2/M checkpoints), DNA repair, apoptosis, and cellular differentiation by interacting with cell cycle proteins (e.g., CDKs, PCNA) and signaling pathways (e.g., p38 MAPK, JNK). Its role in tumor suppression, particularly in silencing certain cancers through promoter hypermethylation, has drawn significant research interest.
The GADD45G antibody enables detection and quantification of endogenous GADD45G protein levels in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use it to explore GADD45G's expression patterns under stress conditions, its interplay with p53-dependent/independent pathways, and its involvement in diseases like cancer, neurodegenerative disorders, and immune dysregulation. Validated antibodies with high specificity are crucial, as GADD45 family members (GADD45A, GADD45B, GADD45G) share structural homology. Recent studies also investigate its potential as a biomarker or therapeutic target, emphasizing the antibody's utility in both basic research and translational applications.
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