| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | G13 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GNA13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GNA13抗体的3篇参考文献示例(注：文献信息为模拟，实际引用需核实)：
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1. **文献名称**: *GNA13 promotes tumor cell invasion and metastasis in colorectal cancer via activation of RhoA signaling*
**作者**: Shi, Y., Chen, L., & Zhou, J.
**摘要**: 本研究探讨了GNA13在结直肠癌转移中的作用，利用特异性GNA13抗体进行Western blot和免疫组化分析。结果显示，GNA13通过激活RhoA信号通路增强肿瘤细胞侵袭能力，提示其作为转移抑制潜在靶点。
2. **文献名称**: *GNA13 regulates epithelial-mesenchymal transition in breast cancer through YAP/TAZ activation*
**作者**: Chen, X., Wang, H., & Liu, Q.
**摘要**: 通过免疫沉淀(使用GNA13抗体)和基因敲除实验，研究发现GNA13通过调控YAP/TAZ通路促进乳腺癌细胞的EMT过程，揭示了其在肿瘤微环境重塑中的关键机制。
3. **文献名称**: *Antibody-based validation of GNA13 as a therapeutic target in hepatocellular carcinoma*
**作者**: Zhang, R., Li, M., & Gao, S.
**摘要**: 该研究开发并验证了一种高特异性GNA13抗体，用于肝细胞癌组织芯片分析。结果显示GNA13高表达与患者预后不良相关，为靶向治疗提供了实验依据。
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**备注**：以上文献信息为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献，并核对作者、期刊及摘要内容。
**Background of GNA13 Antibody**
GNA13 (Guanine Nucleotide-Binding Protein Subunit Alpha-13) is a member of the Gα12/13 subfamily of heterotrimeric G-protein alpha subunits, which play critical roles in transmitting signals from G-protein-coupled receptors (GPCRs) to intracellular effector pathways. GNA13 is involved in regulating diverse cellular processes, including cell proliferation, migration, cytoskeletal reorganization, and apoptosis, primarily through activation of Rho GTPase signaling pathways such as RhoA. Dysregulation of GNA13 has been implicated in cancer progression, cardiovascular diseases, and immune disorders, with studies linking its overexpression or hyperactivation to enhanced tumor invasiveness, metastasis, and poor clinical outcomes.
GNA13 antibodies are essential tools for investigating the expression, localization, and functional roles of GNA13 in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to study protein levels, tissue distribution, and interaction partners. Specificity is a key consideration, as GNA13 shares homology with other Gα subunits (e.g., GNA12), necessitating rigorous validation via knockout controls or siRNA-based silencing. Some antibodies target unique epitopes within conserved domains, such as the GTP-binding region or C-terminal tail, to ensure precise detection.
Research utilizing GNA13 antibodies has advanced understanding of its role in diseases and highlighted its potential as a therapeutic target, particularly in cancers with aberrant GPCR-Rho signaling. Reliable antibodies remain crucial for unraveling the complex mechanisms governed by GNA13 in health and disease.
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