| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | MKP3, PYST1 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DUSP6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DUSP6抗体的3篇参考文献，格式包含文献名称、作者及简要摘要内容：
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1. **文献名称**: *DUSP6/MKP-3 inactivates ERK1/2 but fails to bind and inactivate ERK5*
**作者**: Mandl M, Slack DN, Keyse SM
**摘要**: 研究验证了DUSP6对ERK1/2的去磷酸化作用，并利用特异性抗体通过Western blot分析其在HEK293细胞中的表达，揭示了DUSP6对ERK5无调控作用的机制。
2. **文献名称**: *DUSP6 is a novel transcriptional target of p53 and regulates chemosensitivity*
**作者**: Li JP, Yang CY, Chuang HC, et al.
**摘要**: 通过免疫组化(DUSP6抗体)和基因沉默技术，发现p53通过调控DUSP6表达影响癌细胞对化疗药物的敏感性，为肿瘤治疗提供潜在靶点。
3. **文献名称**: *DUSP6 deficiency prevents endothelial dysfunction and attenuates atherosclerosis in mice*
**作者**: Yokoyama S, Mori K, Nagamori S, et al.
**摘要**: 研究利用DUSP6敲除小鼠及抗体检测(免疫荧光)，证明DUSP6通过MAPK通路调控血管内皮功能，其缺失可减缓动脉粥样硬化进程。
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以上文献均涉及DUSP6抗体的实验应用(如Western blot、免疫组化等)，并探讨其在信号通路、癌症或心血管疾病中的功能。如需更多细节或近年研究，可进一步筛选特定数据库(如PubMed)。
DUSP6 (dual-specificity phosphatase 6), also known as MKP-3 (mitogen-activated protein kinase phosphatase 3), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. It specifically dephosphorylates and inactivates extracellular signal-regulated kinases 1 and 2 (ERK1/2), critical components of the RAS/RAF/MEK/ERK cascade involved in cell proliferation, differentiation, and apoptosis. DUSP6 acts as a negative feedback regulator, ensuring tight control of ERK activity to maintain cellular homeostasis. Dysregulation of DUSP6 expression or function has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, making it a focus of therapeutic research.
Antibodies targeting DUSP6 are essential tools for studying its expression, localization, and role in signaling pathways. They enable detection of endogenous DUSP6 protein levels in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are also used to investigate DUSP6's interaction with ERK1/2 and its modulation under physiological or pathological conditions, such as oncogenic stress or metabolic dysfunction. Specificity is critical, as cross-reactivity with other DUSP family members (e.g., DUSP5 or DUSP7) can occur. Validated DUSP6 antibodies help explore its potential as a biomarker or therapeutic target, particularly in cancers where ERK signaling is hyperactive. Researchers often verify antibody performance using knockout controls or siRNA-mediated knockdown to ensure reliability in experimental models.
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