| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ALDC; ALDH1; PUMB1; ALDH11; RALDH1; ALDH-E1; MGC2318; ALDH1A1 |
| Entrez GeneID | 216 |
| clone | 5A11 |
| WB Predicted band size | 55kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ALDH1A1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是基于ENPP5相关研究领域的示例性参考文献框架(请注意，这些内容为模拟生成，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*Characterization of ENPP5 in Mammalian Testicular Development*
**作者**：Smith J, et al.
**摘要**：本研究通过制备特异性ENPP5抗体，揭示了该酶在小鼠睾丸组织中的高表达模式，并证实其参与精子形成过程中的核苷酸代谢调控，为男性生殖健康研究提供新靶点。
2. **文献名称**：*ENPP5 as a Potential Biomarker in Ovarian Cancer*
**作者**：Chen L, et al.
**摘要**：利用ENPP5单克隆抗体进行免疫组化分析，发现其在卵巢癌组织中表达显著上调，且与患者预后不良相关，提示ENPP5可能作为新型诊断标志物或治疗靶点。
3. **文献名称**：*ENPP5 Antibody-Based Detection in Neural Cell Models*
**作者**：Yamamoto K, et al.
**摘要**：通过开发高亲和力ENPP5抗体，本研究验证了其在神经元细胞中的定位及功能，证明ENPP5通过水解胞外ATP调节神经突触可塑性，为神经系统疾病机制提供新见解。
---
**建议用户**：
- 使用关键词 `ENPP5 antibody application`、`ENPP5 function` 在 **PubMed** 或 **Google Scholar** 检索最新文献。
- 筛选时关注抗体开发、疾病机制或分子功能研究类论文，注意结合研究领域(如癌症、生殖生物学等)缩小范围。
ENPP5. a member of the ectonucleotide pyrophosphatase/phosphodiesterase (ENPP) family, is a transmembrane glycoprotein involved in nucleotide metabolism and cellular signaling. It hydrolyzes extracellular nucleotides like ATP and NAD+ to regulate purinergic signaling pathways, which influence processes such as inflammation, apoptosis, and cell differentiation. ENPP5 is notably expressed in reproductive tissues, particularly the testis, where it may play roles in sperm maturation and fertilization. Its unique substrate specificity and tissue distribution distinguish it from other ENPP family members (e.g., ENPP1-3).
ENPP5 antibodies are critical tools for studying its expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. They enable applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ENPP5 in tissue sections or cell lysates. Research using ENPP5 antibodies has explored its potential roles in male infertility, cancer (e.g., modulating tumor microenvironments via nucleotide signaling), and metabolic disorders. However, commercial availability remains limited, and validation for specificity (e.g., knockout controls) is essential due to homology within the ENPP family. Ongoing studies aim to clarify ENPP5's mechanistic contributions to disease and its utility as a therapeutic target.
×
