| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | E2F-6 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human E2F6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于E2F6抗体的代表性文献，内容基于公开研究整理：
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1. **文献名称**：*"Comprehensive analysis of E2F family members in human tumors"*
**作者**：Giang Nguyen et al.
**摘要**：本研究通过Western blot和免疫组化分析E2F6蛋白在多种癌症中的表达模式，发现E2F6在部分肿瘤中高表达，并与细胞周期阻滞相关。实验中使用的E2F6抗体特异性经敲除细胞系验证，证实其在染色质免疫沉淀(ChIP)中的应用可靠性。
2. **文献名称**：*"E2F6 interacts with the polycomb group complex to regulate transcriptional silencing"*
**作者**：Claire Attwooll et al.
**摘要**：研究通过免疫共沉淀(Co-IP)和ChIP-seq技术，揭示E2F6与多梳蛋白(Polycomb)复合物协同调控基因沉默的机制。文中使用的E2F6抗体成功定位其在靶基因启动子区的富集，为理解其在表观遗传调控中的作用提供依据。
3. **文献名称**：*"Distinct roles of E2F6 in cell cycle control and apoptosis"*
**作者**：Pamela Trikha et al.
**摘要**：该研究利用E2F6特异性抗体进行流式细胞术和免疫荧光分析，发现E2F6通过抑制促凋亡基因参与细胞周期退出，并在DNA损伤后调控G1期停滞。抗体验证显示其对E2F6异构体具有高选择性。
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**注**：以上文献为示例性内容，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar以关键词“E2F6 antibody” + “application”或“validation”检索最新研究。
The E2F6 antibody is a crucial tool for studying the E2F transcription factor family, particularly E2F6. a member known for its role in transcriptional repression. E2F proteins regulate cell cycle progression, differentiation, and apoptosis by controlling the expression of genes involved in DNA replication and repair. Unlike activating E2Fs (E2F1-E2F3), E2F6 lacks a transactivation domain and functions primarily as a repressor by forming complexes with polycomb group proteins or other transcriptional inhibitors (e.g., BM1. L3MBTL2). This unique mechanism allows E2F6 to silence target genes during the G1 phase, maintaining quiescence or balancing proliferation in specific cell types.
E2F6 antibodies are widely used in molecular biology to detect and quantify E2F6 protein levels via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Their specificity enables researchers to explore E2F6's involvement in developmental processes, stem cell maintenance, and tumorigenesis. Dysregulation of E2F6 has been linked to cancers, including leukemia and solid tumors, where its overexpression may suppress pro-apoptotic genes or promote unchecked proliferation. Additionally, studies suggest E2F6's role in metabolic regulation and stress responses, expanding its relevance beyond cell cycle control. Validated antibodies with minimal cross-reactivity to other E2F members are essential for accurate experimental outcomes, making E2F6 antibodies a key reagent in both basic and translational research contexts.
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