| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CREB |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CREB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CREB1抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*CREB1 phosphorylation and transcriptional activity in neuronal plasticity*
**作者**：Ginty DD, et al.
**摘要**：研究通过Western blot和免疫荧光技术，使用特异性CREB1抗体验证其在海马神经元活性依赖性磷酸化中的作用，揭示了CREB1在突触可塑性中的关键调控机制。
2. **文献名称**：*Genome-wide analysis of CREB1 binding sites using ChIP-seq*
**作者**：Lonze BE, Riccio A
**摘要**：利用CREB1抗体进行染色质免疫沉淀测序(ChIP-seq)，鉴定了CREB1在小鼠肝脏细胞中的全基因组结合位点，揭示了其与代谢相关基因的调控关联。
3. **文献名称**：*Antibody-specific validation for CREB1 in human cancer tissues*
**作者**：Mayr B, Montminy M
**摘要**：通过免疫组化(IHC)和siRNA敲除实验，验证了CREB1抗体在多种癌症组织中的特异性，并发现CREB1高表达与肿瘤侵袭性相关。
**注意**：以上文献信息为模拟示例，实际引用时需检索PubMed或Google Scholar获取真实文献。
CREB1 (cAMP Response Element-Binding Protein 1) is a critical transcription factor involved in regulating gene expression in response to various cellular signals, including cAMP, calcium, and growth factors. It binds to cAMP response elements (CREs) in DNA, modulating the transcription of target genes essential for processes like neuronal plasticity, memory formation, circadian rhythm regulation, and cell survival. Dysregulation of CREB1 has been implicated in neurological disorders, cancer, and metabolic diseases.
CREB1 antibodies are widely used in research to detect and quantify CREB1 protein levels, localization, and post-translational modifications (e.g., phosphorylation at Ser133. which activates its transcriptional activity). These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is crucial, as CREB1 shares homology with related family members (e.g., CREM, ATF1). High-quality antibodies are validated using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity.
Applications span studying CREB1's role in synaptic plasticity, stress responses, and disease mechanisms. For instance, in neuroscience, CREB1 antibodies help explore its contribution to learning and memory, while in cancer research, they assess its involvement in tumor progression. Commercial antibodies may target distinct epitopes (N-terminal, C-terminal, or phosphorylated regions), allowing researchers to probe specific functional states. Proper validation and optimization for each experimental system are emphasized to ensure reliable data.
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