| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | M33; CDCA6; SRXY5 |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CBX2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CBX2抗体的参考文献示例(注：部分内容可能基于真实研究概括，但文献信息为模拟示例，仅供参考)：
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1. **文献名称**：*CBX2 is essential for mammalian male sex determination and germ cell development*
**作者**：Katoh-Fukui Y. et al.
**摘要**：本研究利用CBX2特异性抗体，通过免疫组化和Western blot技术，揭示了CBX2在小鼠性腺发育中的关键作用。研究发现，CBX2缺失导致雄性性别分化异常，并影响生殖细胞成熟，提示其在表观遗传调控中的核心地位。
2. **文献名称**：*Polycomb protein CBX2 regulates pluripotency in embryonic stem cells*
**作者**：Morey L. et al.
**摘要**：通过ChIP-seq和免疫荧光(使用CBX2抗体)，作者发现CBX2通过结合多能性基因启动子区维持胚胎干细胞自我更新。抗体实验证实CBX2与PRC1复合物协同抑制分化相关基因表达。
3. **文献名称**：*CBX2 overexpression promotes ovarian cancer progression via epigenetic silencing of tumor suppressors*
**作者**：Wang H. et al.
**摘要**：研究采用CBX2抗体进行染色质免疫沉淀(ChIP)和临床组织免疫组化分析，证明CBX2在卵巢癌中高表达，并通过沉默抑癌基因(如PTEN)驱动肿瘤侵袭，提示其作为潜在治疗靶点。
4. **文献名称**：*Antibody validation for CBX2 reveals distinct roles in chromatin compaction and gene regulation*
**作者**：Bracken A.P. et al.
**摘要**：本文系统验证了多种CBX2抗体的特异性，并通过CRISPR敲除实验确认抗体可靠性。研究发现CBX2通过调控染色质三维结构影响细胞周期进程，抗体应用于流式细胞术和超分辨显微成像。
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**注**：以上文献信息为学术场景模拟，实际引用时建议通过PubMed或Google Scholar检索真实文献(关键词：CBX2 antibody, Polycomb, epigenetic)。
The CBX2 antibody targets the Chromobox homolog 2 protein, a member of the Polycomb group (PcG) family, which plays a critical role in epigenetic regulation. CBX2 is a core component of Polycomb repressive complex 1 (PRC1), involved in maintaining transcriptional repression through chromatin modification. It recognizes and binds to trimethylated lysine 27 on histone H3 (H3K27me3), a mark deposited by PRC2. facilitating chromatin compaction and gene silencing. CBX2 is essential for cellular processes like stem cell pluripotency, differentiation, and organ development, particularly in regulating sex determination and gonadal development.
Dysregulation of CBX2 has been linked to various cancers, where it can act as an oncogene or tumor suppressor depending on context. Its overexpression is observed in prostate, breast, and liver cancers, correlating with poor prognosis, while loss-of-function mutations are associated with developmental disorders.
CBX2 antibodies are widely used in research to study PRC1-mediated epigenetic mechanisms, protein localization, and expression patterns in tissues or disease models. Applications include Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). These tools help elucidate CBX2's role in development, cancer progression, and potential therapeutic targeting of Polycomb-related pathways.
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