| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TNNI3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TNNI3抗体的参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: "Specificity of TNNI3 Antibodies in Detecting Cardiac Troponin I in Human Myocardium"
**作者**: Smith J, et al.
**摘要**: 研究验证了多种TNNI3抗体在人类心肌组织中的特异性，通过免疫印迹和免疫组化证实其不与骨骼肌肌钙蛋白I交叉反应，为心肌损伤的病理诊断提供了可靠工具。
2. **文献名称**: "TNNI3 Knockout Mice Reveal Essential Role in Cardiac Development"
**作者**: Lee H, et al.
**摘要**: 利用TNNI3特异性抗体分析基因敲除小鼠模型，发现TNNI3缺失导致胚胎期心肌收缩异常，证实其在心脏发育中的关键作用。
3. **文献名称**: "Autoantibodies Against TNNI3 in Dilated Cardiomyopathy Patients"
**作者**: Wang Y, et al.
**摘要**: 通过ELISA和蛋白质芯片技术，检测扩张型心肌病患者血清中抗TNNI3自身抗体，提示其可能与疾病进展和免疫调节异常相关。
4. **文献名称**: "Comparative Study of Commercial TNNI3 Antibodies in Forensic Diagnostics"
**作者**: Gonzalez R, et al.
**摘要**: 评估了市售TNNI3抗体在法医尸检心肌梗死诊断中的敏感性和稳定性，为标准化检测流程提供了数据支持。
(注：以上文献为示例，实际引用需查询PubMed、Web of Science等数据库获取真实研究。)
**Background of TNNI3 Antibody**
The TNNI3 antibody targets troponin I type 3 (TNNI3), a protein encoded by the *TNNI3* gene, which is exclusively expressed in cardiac muscle. TNNI3 is a critical component of the troponin complex, regulating calcium-mediated interactions between actin and myosin during cardiac muscle contraction. As a specific isoform of troponin I, it plays a pivotal role in maintaining proper cardiac function by modulating the sensitivity of myofilaments to calcium ions.
Antibodies against TNNI3 are widely used in cardiovascular research and diagnostics. They help detect and quantify TNNI3 protein levels in tissues or serum, aiding in the study of cardiac diseases such as hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), and myocardial infarction. Mutations in *TNNI3* are linked to inherited cardiomyopathies, making TNNI3 antibodies valuable for investigating genetic variants and their pathological mechanisms.
In clinical settings, TNNI3 antibodies are employed in immunoassays (e.g., ELISA, Western blot, immunohistochemistry) to measure cardiac troponin I (cTnI), a gold-standard biomarker for myocardial injury. Elevated cTnI levels in blood indicate acute cardiac damage, guiding diagnosis and treatment.
Commercial TNNI3 antibodies are typically validated for specificity and sensitivity across species, including humans and model organisms. Their applications extend to studying cardiac development, disease modeling in transgenic animals, and evaluating therapeutic interventions targeting cardiac dysfunction.
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