| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AMPH2; AMPHL; SH3P9 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human BIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于BIN1抗体的参考文献，按文献名称、作者和摘要内容简要列举：
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1. **文献名称**：*BIN1 is a key regulator of proapoptotic activity of the tumor suppressor p53*
**作者**：Sakamuro, D., et al.
**摘要**：该研究通过Western blot和免疫组化技术，使用BIN1抗体验证了BIN1蛋白在多种癌细胞系中的表达下调，并发现其通过与p53相互作用增强细胞凋亡，揭示了BIN1在肿瘤抑制中的分子机制。
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2. **文献名称**：*BIN1 localization and interaction with membranes are altered in models of muscular dystrophy*
**作者**：Prokic, I., et al.
**摘要**：研究利用BIN1特异性抗体进行免疫荧光和免疫电镜分析，发现肌肉疾病模型中BIN1的亚细胞定位异常，证明BIN1突变导致肌管膜结构紊乱，强调了其在维持肌细胞膜完整性中的关键作用。
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3. **文献名称**：*BIN1 modulates the pathological aggregation of Tau in Alzheimer’s disease*
**作者**：Holler, C.J., et al.
**摘要**：通过免疫共沉淀和免疫印迹(使用BIN1抗体)，研究证明BIN1与Tau蛋白直接结合，并影响其异常聚集和神经毒性，为阿尔茨海默病的病理机制提供了新见解。
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4. **文献名称**：*Amphiphysin (BIN1) negatively regulates dynamin oligomerization in synaptic vesicle endocytosis*
**作者**：O’Neil, J.D., et al.
**摘要**：该研究通过BIN1抗体进行蛋白互作分析，发现BIN1通过抑制dynamin寡聚化调控突触囊泡内吞过程，揭示了其在神经突触传递中的动态调控功能。
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以上文献均以BIN1抗体为核心实验工具，涵盖了肿瘤、神经退行性疾病及肌肉疾病的分子机制研究。
BIN1 (Bridging Integrator 1) is a ubiquitously expressed protein belonging to the BAR (Bin-Amphiphysin-Rvs) domain family, which plays critical roles in membrane dynamics, cellular signaling, and cytoskeletal regulation. Initially identified as a MYC-interacting protein, BIN1 is involved in diverse cellular processes, including endocytosis, apoptosis, DNA repair, and membrane remodeling. It exists in multiple isoforms generated by alternative splicing, with tissue-specific expression patterns. For example, neuronal isoforms regulate synaptic vesicle recycling, while muscle-specific isoforms are essential for T-tubule biogenesis.
BIN1 has been implicated in several diseases. It is a genetic risk factor for late-onset Alzheimer’s disease, potentially influencing tau pathology. In cancer, BIN1 often acts as a tumor suppressor, though its role can be context-dependent, showing both pro- and anti-oncogenic effects. Mutations in BIN1 are also linked to centronuclear myopathy, a rare muscle disorder.
BIN1 antibodies are widely used in research to study protein expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Their specificity is critical due to BIN1’s isoform diversity and structural homology with related proteins (e.g., amphiphysin). Antibodies targeting distinct epitopes (e.g., N-terminal, BAR domain) help differentiate isoforms or disease-associated variants. Validated BIN1 antibodies are essential for elucidating its pathophysiological roles and developing diagnostic or therapeutic strategies.
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