| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human KRT81 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KRT81抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"KRT81 expression in triple-negative breast cancer: Association with epithelial-mesenchymal transition and therapeutic implications"*
**作者**：Chen L, et al.
**摘要**：本研究通过免疫组化(IHC)和Western blot分析，发现KRT81在三阴性乳腺癌(TNBC)中高表达，并与上皮-间充质转化(EMT)标志物(如E-cadherin和Vimentin)显著相关。KRT81抗体的应用揭示了其在预测TNBC患者预后及化疗耐药性中的潜在作用。
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2. **文献名称**：*"Characterization of a novel monoclonal antibody against human KRT81 for pathological diagnosis"*
**作者**：Tanaka K, et al.
**摘要**：作者开发了一种针对KRT81的新型单克隆抗体，并通过免疫荧光和流式细胞术验证其特异性。该抗体成功应用于皮肤鳞状细胞癌(SCC)的病理组织检测，证实KRT81在肿瘤侵袭边缘的高表达可能成为诊断标志物。
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3. **文献名称**：*"KRT81 mutations disrupt hair shaft integrity and cause autosomal recessive hypotrichosis"*
**作者**：Shimomura Y, et al.
**摘要**：研究利用KRT81抗体对遗传性毛发稀疏症患者进行蛋白定位分析，发现KRT81基因突变导致角蛋白纤维结构异常，破坏毛干强度。该研究为KRT81在毛发疾病中的功能提供了直接证据。
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4. **文献名称**：*"KRT81 as a biomarker for circulating tumor cells in colorectal cancer"*
**作者**：Wang H, et al.
**摘要**：通过免疫磁珠分离技术结合KRT81抗体，研究者从结直肠癌患者血液中高效捕获循环肿瘤细胞(CTCs)，并证实KRT81阳性CTCs与转移风险增加相关，为肿瘤液体活检提供了新策略。
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以上文献涵盖了KRT81抗体在癌症诊断、毛发疾病机制及抗体开发中的应用，涉及基础研究与临床转化方向。
The KRT81 antibody targets keratin 81. a protein encoded by the *KRT81* gene, which belongs to the type II keratin family of intermediate filament proteins. Keratins are crucial structural components of epithelial cells, providing mechanical stability and resilience to tissues. Specifically, KRT81 pairs with type I keratins (e.g., KRT31. KRT35) to form heterodimers that assemble into filaments, predominantly expressed in hair follicles, nails, and other specialized epithelial structures. This keratin plays a vital role in maintaining the integrity of hair shafts and cuticles.
Antibodies against KRT81 are widely used in research to study hair biology, genetic disorders affecting keratinization, and epithelial differentiation. They are essential tools in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to localize and quantify KRT81 expression in tissues or cell lines. Dysregulation or mutations in *KRT81* have been linked to hair disorders, such as monilethrix and other congenital hair shaft abnormalities. Additionally, KRT81 overexpression has been observed in certain cancers, suggesting potential roles in tumor progression or epithelial-mesenchymal transition.
Commercial KRT81 antibodies are typically developed in hosts like rabbits or mice, validated for specificity through knockdown/knockout controls. Their applications span basic research, diagnostic pathology, and therapeutic target validation, underscoring their importance in dermatological and oncological studies.
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