| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BAK; CDN1; BCL2L7; BAK-LIKE |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human BAK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BAK1抗体的3篇代表性文献，包含标题、作者及摘要概述：
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1. **文献名称**：*Pro-apoptotic BAX and BAK: A requisite gateway to mitochondrial dysfunction and death*
**作者**：Wei, M.C., Zong, W.X., Cheng, E.H., Lindsten, T., Panoutsakopoulou, V., Ross, A.J., et al.
**摘要**：该研究通过基因敲除实验证实BAK1与BAX协同调控线粒体外膜透化(MOMP)，启动细胞色素c释放和凋亡。研究使用BAK1抗体检测蛋白活化状态，表明其寡聚化是凋亡执行的关键步骤。
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2. **文献名称**：*BID-induced structural changes in BAK promote apoptosis*
**作者**：Moldoveanu, T., Liu, Q., Tocilj, A., Watson, M., Shore, G., Gehring, K.
**摘要**：通过X射线晶体学解析BAK1蛋白的构象变化，揭示促凋亡因子BID激活BAK1的分子机制。研究利用BAK1特异性抗体验证其在凋亡信号中的构象变化，为靶向药物设计提供结构基础。
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3. **文献名称**：*The role of BAK1 in cancer: Implications for therapy*
**作者**：Coultas, L., Strasser, A.
**摘要**：综述BAK1在肿瘤发生中的双重角色，强调其作为促凋亡蛋白的调控机制及耐药性关联。文中讨论了基于BAK1抗体的检测方法在评估癌症患者化疗敏感性中的应用潜力。
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这些文献涵盖了BAK1的功能机制、结构研究及临床相关性，抗体应用场景包括蛋白检测、构象分析和治疗评估。
BAK1 (BRI1-Associated Receptor Kinase 1), also known as SERK3 (Somatic Embryogenesis Receptor Kinase 3), is a leucine-rich repeat receptor-like kinase (LRR-RLK) critical in plant signaling pathways. Initially identified as a co-receptor for brassinosteroid (BR) signaling through interaction with the BR receptor BRI1. BAK1 is now recognized as a multifunctional regulator involved in plant development, immune responses, and cell death regulation. It forms dynamic complexes with multiple pattern recognition receptors (PRRs), such as FLS2 and EFR, to amplify immune signaling upon pathogen-associated molecular pattern (PAMP) perception.
BAK1 antibodies are essential tools for studying its protein expression, localization, and interaction networks. These antibodies, often generated against specific epitopes in the kinase domain or extracellular LRR region, enable detection via Western blot, immunoprecipitation, or microscopy. Researchers use them to explore BAK1's dual role in BR-mediated growth and pathogen defense, particularly its involvement in regulating reactive oxygen species (ROS) production and stomatal immunity.
Studies employing BAK1 antibodies have revealed its phosphorylation-dependent activation mechanisms and crosstalk with other signaling components. Knockout mutants (e.g., *bak1* in *Arabidopsis*) and antibody-based assays help dissect its contributions to plant-microbe interactions and stress adaptation. Validations typically include specificity checks in mutant backgrounds. BAK1 antibodies thus remain pivotal in unraveling the molecular basis of plant receptor kinase signaling and its agricultural applications.
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