Rabbit Polyclonal NFKBIB Antibody

The NFKBIB antibody is a crucial tool for studying the NF-κB signaling pathway, which regulates immune responses, inflammation, and cell survival. NFKBIB (Nuclear Factor Kappa B Inhibitor Beta), also known as IκBβ, is an inhibitory protein that binds to NF-κB transcription factors, sequestering them in the cytoplasm and preventing their activation. Upon stimuli like cytokines or stress, IκB proteins undergo phosphorylation and degradation, releasing NF-κB to translocate into the nucleus and initiate gene expression. NFKBIB antibodies are widely used in research to detect and quantify endogenous NFKBIB protein levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate NFKBIB’s regulatory roles in diseases such as cancer, autoimmune disorders, and chronic inflammation. Specificity is critical; many NFKBIB antibodies are validated using knockout cell lines or siRNA knockdown to ensure accurate detection. Monoclonal antibodies offer high consistency, while polyclonal versions may detect multiple epitopes. Researchers rely on NFKBIB antibodies to explore post-translational modifications, protein interactions, and subcellular localization, aiding in the development of therapeutic strategies targeting NF-κB dysregulation. Proper controls and validation are essential to avoid cross-reactivity with related IκB family members like IκBα (NFKBIA) or IκBε (NFKBIE).