| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | COXVB |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human COX5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COX5B抗体的模拟参考文献示例(注：文献为示例性质，实际文献需通过学术数据库查询)：
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1. **标题**: "Cytochrome c oxidase subunit 5B (COX5B) as a prognostic marker in colorectal cancer"
**作者**: Smith A, et al.
**摘要**: 研究通过COX5B抗体检测结直肠癌组织中线粒体COX5B蛋白表达水平，发现其低表达与患者生存率降低相关，提示COX5B可能作为预后生物标志物。
2. **标题**: "COX5B regulates oxidative phosphorylation and mitochondrial reactive oxygen species production"
**作者**: Lee JH, et al.
**摘要**: 利用COX5B抗体进行蛋白质相互作用分析，揭示COX5B通过调控复合体IV活性影响线粒体ROS生成，在代谢疾病中起关键作用。
3. **标题**: "Antibody-based profiling of COX5B in Alzheimer's disease brain tissues"
**作者**: Garcia R, et al.
**摘要**: 通过免疫组化(COX5B抗体)发现阿尔茨海默病患者脑组织中COX5B表达异常，可能与线粒体功能障碍及神经元退行性病变相关。
4. **标题**: "Development and validation of a COX5B-specific monoclonal antibody for mitochondrial studies"
**作者**: Chen X, et al.
**摘要**: 研究报道了一种高特异性COX5B单克隆抗体的开发，验证了其在Western blot及免疫荧光中对多种物种线粒体样本的适用性。
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建议通过 **PubMed** 或 **Google Scholar** 搜索关键词“COX5B antibody”或“COX5B function”获取真实文献。
The COX5B antibody is a crucial tool in biomedical research for studying cytochrome c oxidase (COX), also known as Complex IV, a key enzyme in the mitochondrial electron transport chain. COX catalyzes the final step of oxidative phosphorylation, transferring electrons to oxygen to form water while driving ATP synthesis. The COX enzyme comprises multiple subunits, with COX5B being a nuclear-encoded subunit critical for stabilizing the complex's structure and function.
COX5B antibodies are specifically designed to detect and quantify the COX5B protein in various experimental settings, such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies enable researchers to investigate COX5B expression levels, localization, and interactions in different tissues or disease models. Dysregulation of COX5B has been implicated in mitochondrial disorders, cancer, and neurodegenerative diseases, making its study vital for understanding metabolic dysfunction.
Commercial COX5B antibodies are typically raised in hosts like rabbits or mice using immunogenic peptide sequences unique to COX5B. Validation includes testing for specificity via knockout controls or siRNA knockdown. Researchers use these antibodies to explore mitochondrial bioenergetics, cellular responses to hypoxia, and metabolic adaptations in pathologies. Their applications extend to diagnostics and therapeutic development, particularly in diseases linked to oxidative phosphorylation defects.
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