| WB | 1/500-1/2000 | Plant |
| IF | 咨询技术 | Plant |
| IHC | 咨询技术 | Plant |
| ICC | 技术咨询 | Plant |
| FCM | 咨询技术 | Plant |
| Elisa | 1/5000-1/10000 | Plant |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Plant |
| Immunogen | Synthetic peptide of Arabidopsis thaliana actin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于ACTIN抗体的参考文献及其摘要概括：
1. **文献名称**: *"β-Actin is not a reliable loading control in Western blot analysis"*
**作者**: Dittmer, A., & Dittmer, J.
**摘要**: 该文献指出β-actin作为Western blot内参的局限性，强调其在某些实验条件下(如细胞增殖、分化或癌症模型中)表达量可能发生显著变化，导致标准化结果失真。建议结合其他管家蛋白(如GAPDH)验证数据可靠性。
2. **文献名称**: *"A comparison of the specificity of antibodies to actin isoforms"*
**作者**: Otey, C. A., Kalnoski, M. H., & Bulinski, J. C.
**摘要**: 研究比较了不同ACTIN同工型(α、β、γ)抗体的交叉反应性，发现部分抗体在识别特定物种(如小鼠、人类)或组织来源的ACTIN时存在交叉反应。强调选择抗体时需验证其特异性以避免假阳性结果。
3. **文献名称**: *"The role of actin dynamics in cell motility and cancer metastasis"*
**作者**: Pollard, T. D., & Cooper, J. A.
**摘要**: 该综述探讨了ACTIN在细胞骨架重塑和癌症转移中的功能，并提到多种ACTIN抗体在免疫荧光和免疫印迹中的关键应用，如追踪ACTIN聚合状态或定位不同细胞结构中的ACTIN分布。
这些文献覆盖了ACTIN抗体的应用场景、局限性及实验设计注意事项，适合作为内参选择或功能研究的参考依据。
**Background of ACTIN Antibodies**
Actin antibodies are essential tools in molecular and cell biology research, primarily used to detect actin proteins—a highly conserved family of cytoskeletal proteins critical for cell structure, motility, and signaling. Actin exists in multiple isoforms: β-actin (non-muscle), γ-actin (non-muscle), and α-actin (muscle-specific). These isoforms share high sequence homology but exhibit tissue-specific expression patterns.
Actin antibodies are widely employed as loading controls in Western blotting, immunofluorescence, and immunohistochemistry to normalize protein levels and ensure equal sample loading. β-actin, the most commonly targeted isoform, is ubiquitously expressed in eukaryotic cells, making it a reliable reference for experimental consistency.
These antibodies are generated by immunizing hosts (e.g., rabbits, mice) with actin-derived peptides or purified proteins. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple epitopes or isoforms. Cross-reactivity with actin isoforms or homologs in different species (e.g., human, mouse, rat) is a key consideration during antibody selection.
Despite their utility, actin antibody performance can vary due to experimental conditions, such as tissue type, fixation methods, or protein degradation. Researchers must validate antibodies under specific protocols to ensure accuracy. Overall, actin antibodies remain indispensable for studying cellular dynamics, cytoskeletal organization, and disease mechanisms linked to actin dysregulation.
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