| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32 |
| Entrez GeneID | 28977 |
| clone | 3H6H2 |
| WB Predicted band size | 16.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MRPL42 (AA: 10-142) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MRPL42抗体的3篇参考文献的简要概括：
1. **文献名称**: *Mitochondrial Ribosome Assembly in Health and Disease*
**作者**: Richter-Dennerlein R, et al.
**摘要**: 该研究通过免疫印迹(Western blot)和免疫荧光技术，使用MRPL42抗体分析线粒体核糖体大亚基的组装过程，揭示了MRPL42在维持线粒体翻译功能中的关键作用，并探讨其突变与线粒体疾病的相关性。
2. **文献名称**: *MRPL42 Overexpression Predicts Poor Prognosis in Colorectal Cancer*
**作者**: Lee S, et al.
**摘要**: 研究利用MRPL42抗体对结直肠癌组织进行免疫组化分析，发现MRPL42的高表达与肿瘤转移和患者生存率降低显著相关，提示其可能作为癌症预后的生物标志物。
3. **文献名称**: *Oxidative Stress Alters Mitochondrial Ribosomal Protein Composition*
**作者**: Soto-Heredro G, et al.
**摘要**: 通过MRPL42抗体检测发现，氧化应激条件下细胞线粒体核糖体蛋白(包括MRPL42)表达下调，表明线粒体翻译功能受损可能是应激反应的重要机制。
注：上述文献为示例性概括，实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以“MRPL42 antibody”为关键词检索最新文献。
The MRPL42 antibody is a crucial tool for investigating the mitochondrial ribosomal protein L42 (MRPL42), a component of the mitochondrial large ribosomal subunit. Mitochondrial ribosomes are responsible for translating mitochondrial DNA-encoded mRNAs, which are essential for oxidative phosphorylation (OXPHOS) and cellular energy production. MRPL42. encoded by the nuclear genome, is part of the 39S subunit and plays a role in ribosome assembly and stability. Dysregulation of mitochondrial ribosome proteins, including MRPL42. has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases.
Antibodies targeting MRPL42 are widely used in research to study mitochondrial protein synthesis, ribosome biogenesis, and mitochondrial dysfunction. They enable detection of MRPL42 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry, helping to assess tissue-specific expression patterns or alterations in disease models. Recent studies suggest MRPL42 may serve as a biomarker in certain cancers, with overexpression observed in tumor tissues.
Validation of MRPL42 antibodies typically involves knockout controls or siRNA knockdown to confirm specificity. Researchers should verify cross-reactivity across species if applied to non-human models. These antibodies are vital for exploring the molecular basis of mitochondrial diseases and developing therapeutic strategies targeting mitochondrial translation machinery.
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