| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 78995 |
| clone | 7A3A9 |
| WB Predicted band size | 69.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human C17ORF53 (AA: 282-527) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下为示例性参考文献(注：C17ORF53研究较少，具体文献需根据实际数据库验证)：
1. **文献名称**: "Characterization of C17ORF53 as a novel regulator of mitochondrial function"
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫沉淀和Western blot技术，利用C17ORF53抗体发现该蛋白定位于线粒体，并参与调节细胞能量代谢和氧化应激反应。
2. **文献名称**: "C17ORF53 interacts with p53 and modulates DNA damage response"
**作者**: Zhang L, et al.
**摘要**: 通过免疫荧光和共聚焦显微镜技术结合C17ORF53抗体，证实C17ORF53与p53蛋白存在相互作用，影响DNA损伤修复通路及肿瘤细胞凋亡。
3. **文献名称**: "Proteomic analysis of C17ORF53 in breast cancer progression"
**作者**: Johnson R, et al.
**摘要**: 使用C17ORF53抗体进行组织芯片分析，发现其在乳腺癌组织中高表达，并与肿瘤侵袭性及患者预后不良相关。
4. **文献名称**: "C17ORF53 antibody validation for flow cytometry applications"
**作者**: Lee S, et al.
**摘要**: 本文系统验证了多种C17ORF53抗体的特异性及适用性，优化了其在流式细胞术中的检测条件，为免疫表型研究提供工具支持。
**注意**：以上文献信息为模拟示例，实际文献需通过PubMed、Google Scholar等平台以“C17ORF53 antibody”或更新基因名称检索确认。若研究较少，建议扩展至相关通路或蛋白家族文献。
**Background of C17ORF53 Antibody**
C17ORF53 (Chromosome 17 Open Reading Frame 53), also known as CFAP53 or CILD44. is a protein-coding gene located on human chromosome 17q21.2. While its precise molecular function remains under investigation, studies suggest its potential involvement in ciliary assembly and motility, with mutations linked to primary ciliary dyskinesia (PCD), a genetic disorder affecting respiratory and reproductive systems. C17ORF53 antibodies are immunological tools designed to detect and study the expression, localization, and interactions of the C17ORF53 protein. These antibodies are typically produced in host organisms (e.g., rabbits or mice) using immunogenic peptides derived from conserved regions of the protein.
C17ORF53 antibodies are widely utilized in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate protein expression levels in tissues or cell lines. Their applications extend to research on ciliopathies, respiratory diseases, and cellular mechanisms underlying ciliary dysfunction. Validation data, including specificity (e.g., knockout-validated) and cross-reactivity, are critical for ensuring reliability in experimental settings. As research advances, C17ORF53 antibodies continue to support efforts in elucidating the gene’s role in health and disease.
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