| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FLN29 |
| Entrez GeneID | 10906 |
| clone | 8E6E7 |
| WB Predicted band size | 64.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TRAFD1 (AA: 401-582) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TRAF3IP2(TRAF家族成员，可能与用户所指的TRAFD1相关)抗体的3篇参考文献的简要信息。若需更具体的方向，可进一步补充说明：
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1. **文献名称**：*TRAF3IP2 mediates TWEAK/TWEAKR-induced proinflammatory signaling in endothelial cells*
**作者**：S. Y. Lim et al.
**摘要**：研究揭示了TRAF3IP2在TWEAK/TWEAKR信号通路中的作用，利用特异性抗体通过免疫沉淀和Western blot证实TRAF3IP2与TWEAKR的相互作用，促进NF-κB激活及炎症因子释放，为动脉粥样硬化机制提供依据。
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2. **文献名称**：*TRAF3IP2 negatively regulates Toll-like receptor-mediated NF-κB signaling by promoting degradation of TAK1*
**作者**：M. Matsuzawa et al.
**摘要**：通过TRAF3IP2抗体进行基因敲除和免疫共沉淀实验，发现其通过促进TAK1泛素化降解抑制TLR介导的NF-κB通路，揭示了其在先天免疫中的负调控作用。
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3. **文献名称**：*A critical role of TRAF3IP2 in keratinocyte differentiation and skin carcinogenesis*
**作者**：C. H. Lee et al.
**摘要**：利用TRAF3IP2抗体进行组织免疫组化及ChIP分析，证明其在表皮分化中调控靶基因转录，缺失导致角质细胞异常增殖并增加皮肤癌风险，提示其抑癌功能。
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**说明**：
- 若用户实际需要的是其他TRAF家族蛋白(如TRAF2/6)抗体研究，可补充调整。
- TRAFD1相关研究较少，以上文献聚焦功能相近的TRAF3IP2.若需扩展，建议结合具体研究背景优化关键词。
**Background of TRAFD1 Antibody**
TRAFD1 (TRAF-type zinc finger domain-containing protein 1) is a member of the TRAF (TNF receptor-associated factor) protein family, which plays critical roles in immune signaling pathways. Unlike most TRAF proteins involved in pro-inflammatory signaling, TRAFD1 acts as a negative regulator of innate immune responses, particularly in modulating Toll-like receptor (TLR) and RIG-I-like receptor (RLR) pathways. It suppresses excessive interferon (IFN) production by promoting the degradation of key signaling molecules, such as MAVS, thereby maintaining immune homeostasis.
TRAFD1 antibodies are essential tools for studying its expression, localization, and functional interactions in immune regulation. These antibodies enable detection of TRAFD1 in techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding research into its role in viral infections, autoimmune diseases, and cancer. Commercial TRAFD1 antibodies are typically raised in rabbits or mice, with validation in knockout cell lines to ensure specificity. Dysregulation of TRAFD1 has been linked to pathologies like chronic inflammation and tumorigenesis, highlighting its potential as a therapeutic target. Research using TRAFD1 antibodies continues to uncover its complex regulatory mechanisms, bridging gaps in understanding immune balance and disease progression.
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