Mouse Monoclonal MAP1LC3A Antibody

MAP1LC3A (microtubule-associated protein 1 light chain 3 alpha), commonly referred to as LC3A, is a key autophagy-related protein involved in the formation and maturation of autophagosomes, double-membrane vesicles that sequester cytoplasmic components for lysosomal degradation. The LC3 protein exists in two forms: cytosolic LC3-I and lipidated, membrane-bound LC3-II, which is a hallmark of autophagosome membranes. Antibodies targeting MAP1LC3A are widely used as critical tools to monitor autophagy flux in cellular and tissue studies. These antibodies enable the detection of LC3-II via techniques like western blotting, immunofluorescence, or immunohistochemistry, helping researchers assess autophagic activity under various conditions, such as nutrient deprivation, stress, or disease states. Due to the dynamic nature of autophagy, proper experimental controls (e.g., using lysosomal inhibitors) are essential to distinguish between increased autophagosome synthesis and impaired degradation. MAP1LC3A antibodies have also been instrumental in studying autophagy's role in cancer, neurodegeneration, infection, and metabolic disorders. However, cross-reactivity with other LC3 isoforms (e.g., LC3B) should be considered when interpreting results. Their specificity and reliability make these antibodies indispensable for advancing our understanding of autophagy mechanisms and their therapeutic implications.