| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PC3; RC1 |
| Entrez GeneID | 57332 |
| clone | 9C3D4 |
| WB Predicted band size | 43.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CBX8 (AA: 17-222) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CBX8抗体的3篇参考文献示例(请注意，以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**：*CBX8 promotes colorectal cancer proliferation and metastasis through activation of Wnt/β-catenin signaling*
**作者**：Zhang Y, et al.
**摘要**：该研究利用CBX8抗体通过免疫组化(IHC)和Western blot分析结直肠癌组织中CBX8的表达，发现其高表达与患者预后不良相关。实验表明，CBX8通过激活Wnt/β-catenin通路促进肿瘤细胞增殖和转移。
2. **文献名称**：*CBX8 maintains stemness in hepatocellular carcinoma by epigenetically repressing differentiation genes*
**作者**：Yuan S, et al.
**摘要**：通过CBX8抗体进行染色质免疫沉淀(ChIP)和免疫荧光染色，研究发现CBX8在肝癌干细胞中特异性高表达，并通过PRC1复合体抑制分化相关基因，维持肿瘤干细胞特性。
3. **文献名称**：*Antibody-based profiling of CBX8 reveals its prognostic value in acute myeloid leukemia*
**作者**：Li H, et al.
**摘要**：该研究开发了一种高特异性CBX8单克隆抗体，用于流式细胞术和免疫组化检测急性髓系白血病(AML)患者样本。结果显示CBX8高表达与化疗耐药性和复发风险升高显著相关。
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**提示**：实际研究中，建议通过PubMed或Google Scholar检索关键词(如“CBX8 antibody”、“CBX8 PRC1 function”)获取真实文献，并注意选择近5年发表的高影响力期刊论文。
The CBX8 antibody is a crucial tool for studying the role of CBX8 (Chromobox Homolog 8), a component of the Polycomb Repressive Complex 1 (PRC1) in the Polycomb group (PcG) protein family. PRC1. along with PRC2. mediates epigenetic gene silencing by modifying chromatin structure. CBX8 contributes to PRC1's function by recognizing histone H2A lysine 119 ubiquitination (H2AK119ub1), a mark deposited by PRC2-mediated H3K27me3. facilitating transcriptional repression. It plays roles in maintaining stem cell pluripotency, cell differentiation, and oncogenesis, with studies implicating CBX8 in cancer progression as a potential oncogene.
CBX8 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions. Due to high homology among CBX family members (CBX2. 4. 6. 7. 8), antibody specificity is critical, requiring validation via knockout controls. Researchers also focus on isoform-specific variants or post-translational modifications of CBX8. which may influence its recruitment to chromatin or partnership with other PRC1 subunits like RING1A/B.
Studies using CBX8 antibodies have revealed its context-dependent roles, such as promoting or suppressing tumors in different cancers, highlighting its therapeutic potential. However, functional redundancy within the CBX family and dynamic PRC1 composition pose challenges, emphasizing the need for precise antibody characterization in experimental models.
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