| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GIG8; ID2A; ID2H; bHLHb26 |
| Entrez GeneID | 3398 |
| clone | 4E12G5 |
| WB Predicted band size | 15kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ID2 (AA: 1-134) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ID2抗体的3篇示例参考文献(虚构内容，仅供格式参考)：
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1. **文献名称**: *ID2 promotes tumor metastasis through EMT regulation in colorectal cancer*
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化(使用ID2抗体)分析结直肠癌组织，发现ID2高表达与上皮-间质转化(EMT)标志物相关，提示其通过激活TGF-β通路促进肿瘤转移。
2. **文献名称**: *ID2 maintains neural stem cell self-renewal via Notch signaling*
**作者**: Johnson B, et al.
**摘要**: 利用ID2抗体进行Western blot和ChIP实验，证明ID2通过抑制分化基因并协同Notch信号通路维持神经干细胞的增殖和未分化状态。
3. **文献名称**: *Targeting ID2 enhances anti-tumor immunity in melanoma*
**作者**: Lee C, et al.
**摘要**: 通过流式细胞术(ID2抗体标记)发现抑制ID2可增强CD8+ T细胞活性，联合免疫检查点抑制剂显著抑制黑色素瘤小鼠模型的肿瘤生长。
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**注意**：以上文献为示例，实际引用需查询PubMed、Google Scholar等数据库获取真实信息。建议结合关键词“ID2 antibody”、“ID2 function”或“ID2 cancer”进行检索。
The ID2 (Inhibitor of DNA Binding 2) antibody is a research tool targeting the ID2 protein, a member of the ID protein family (ID1-4). ID proteins are helix-loop-helix (HLH) transcriptional regulators that lack DNA-binding domains, enabling them to inhibit basic HLH (bHLH) transcription factors by forming non-functional heterodimers. This interaction modulates cellular processes like differentiation, proliferation, and apoptosis.
ID2 is notably involved in development, stem cell maintenance, and oncogenesis. It promotes cell cycle progression by suppressing pRb tumor suppressor activity and is critical in neural, lymphoid, and vascular development. Dysregulation of ID2 is linked to cancers (e.g., breast, prostate, glioblastoma), where its overexpression often correlates with tumor aggressiveness and poor prognosis.
In research, ID2 antibodies are used to detect ID2 expression in tissues or cell lines via techniques like Western blot, immunohistochemistry, and flow cytometry. These studies explore ID2's role in cancer biology, stem cell pluripotency, and tissue regeneration. Recent interest also focuses on its interplay with signaling pathways like TGF-β and BMP. While therapeutic targeting of ID2 remains experimental, its regulatory functions make it a potential biomarker or intervention point in malignancies and regenerative medicine.
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