| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NESTIN |
| Entrez GeneID | 10763 |
| clone | 1A9G12 |
| WB Predicted band size | 177kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NESTIN (AA: 419-588 ) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NES抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *"The human nestin gene: Gene structure, evolution, and expression in human CNS progenitor cells"*
**作者**: Lendahl, U., Zimmerman, L.B., McKay, R.D.
**摘要**: 该研究首次克隆并鉴定了人类NES(巢蛋白)基因，分析了其在神经上皮干细胞中的表达特征。研究表明，NES蛋白是神经前体细胞的重要标记物，其抗体可用于追踪胚胎发育和成体神经干细胞的动态。
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2. **文献名称**: *"Nestin expression correlates with tumor progression in glioblastoma"*
**作者**: Ishiwata, T., Matsuda, Y., Naito, Z.
**摘要**: 研究探讨了NES抗体在胶质母细胞瘤中的诊断价值，发现NES表达水平与肿瘤恶性程度和患者预后相关。通过免疫组化分析，证实NES抗体可用于标记肿瘤干细胞，为癌症治疗提供潜在靶点。
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3. **文献名称**: *"Nestin regulates self-renewal and survival of neural stem cells via Wnt/β-catenin signaling"*
**作者**: Wiese, C., Rolletschek, A., Kania, G.
**摘要**: 该研究利用NES抗体标记小鼠神经干细胞，揭示巢蛋白通过调控Wnt/β-catenin通路维持干细胞的自我更新能力。实验表明，NES表达缺失会导致干细胞分化异常，突显其在神经发育中的关键作用。
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这些文献涵盖了NES抗体的基础研究、肿瘤学应用及分子机制分析，可作为相关领域的研究参考。如需具体DOI或出版信息，可进一步补充。
**Background of NES Antibodies**
NES (Nuclear Export Signal) antibodies are essential tools in studying intracellular protein trafficking, specifically targeting proteins containing nuclear export signals—short amino acid sequences that mediate the transport of molecules from the nucleus to the cytoplasm. These signals are recognized by export receptors, primarily Exportin 1 (CRM1), which facilitates translocation through the nuclear pore complex.
NES antibodies are widely used to investigate the nucleocytoplasmic shuttling of proteins, a process critical for regulating gene expression, cell cycle progression, and stress responses. Dysregulation of nuclear export is implicated in diseases such as cancer, viral infections, and neurodegenerative disorders. For example, certain oncoproteins and viral particles exploit NES-dependent pathways to evade nuclear surveillance or promote proliferation.
In research, NES antibodies help identify export signals in proteins, validate CRM1 interactions (often using inhibitors like leptomycin B), and assess protein localization via techniques like immunofluorescence, Western blotting, or immunoprecipitation. They also contribute to developing therapeutic strategies, such as CRM1 inhibitors in oncology to block aberrant nuclear export of tumor suppressors.
Overall, NES antibodies provide insights into fundamental cellular mechanisms and disease pathways, bridging basic research and clinical applications. Their utility in both mechanistic studies and drug development underscores their importance in molecular and cell biology.
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