| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GLUT4 |
| Entrez GeneID | 6517 |
| clone | 3G10A3 |
| WB Predicted band size | 54.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SLC2A4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Insulin resistance is associated with reduced plasma membrane GLUT4 content in skeletal muscle of patients with type 2 diabetes*
**作者**：Garvey WT, Maianu L, Hancock JA, Golichowski AM
**摘要**：该研究使用SLC2A4抗体(针对GLUT4蛋白)通过Western blot和免疫荧光技术，发现2型糖尿病患者骨骼肌中质膜GLUT4含量显著降低，提示胰岛素抵抗与GLUT4转运障碍相关。
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2. **文献名称**：*Exercise training increases GLUT4 protein expression in obese Zucker rats*
**作者**：Ren JM, Semenkovich CF, Gulve EA, Holloszy JO
**摘要**：通过SLC2A4抗体检测肥胖大鼠骨骼肌和脂肪组织中GLUT4表达水平，发现运动训练可显著上调GLUT4蛋白表达，改善胰岛素敏感性，为运动干预代谢疾病提供依据。
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3. **文献名称**：*Adipose tissue-specific deletion of GLUT4 alters insulin resistance and lipid metabolism*
**作者**：Abel ED, Peroni OD, Kim JK, Kim YB, Boss O, Hadro E
**摘要**：研究利用SLC2A4抗体进行基因敲除小鼠模型的蛋白分析，揭示脂肪组织GLUT4缺失导致全身胰岛素抵抗和血脂异常，强调脂肪细胞GLUT4在代谢调控中的关键作用。
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这些文献均通过SLC2A4抗体探究GLUT4蛋白在胰岛素信号通路、运动干预及代谢疾病中的分子机制，涵盖糖尿病、肥胖等研究领域。
The SLC2A4 antibody targets the solute carrier family 2 member 4 (SLC2A4), also known as glucose transporter type 4 (GLUT4), a critical insulin-regulated transporter responsible for facilitating glucose uptake into adipose and muscle tissues. GLUT4 is stored in intracellular vesicles under basal conditions and translocates to the cell membrane upon insulin stimulation, a process central to maintaining glucose homeostasis. Dysregulation of GLUT4 expression or trafficking is closely associated with insulin resistance, type 2 diabetes, and metabolic syndrome.
SLC2A4 antibodies are widely used in research to detect GLUT4 protein levels, localization, and dynamics in tissues or cell models. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to study GLUT4 membrane translocation, expression changes in metabolic disorders, or responses to therapeutic interventions. These antibodies are often raised in hosts like rabbits or mice, targeting specific epitopes within GLUT4’s cytoplasmic, extracellular, or C-terminal domains.
Validation of SLC2A4 antibodies is essential, as variations in isoform specificity, cross-reactivity, or sensitivity can affect experimental outcomes. Knockout controls or siRNA-mediated silencing are recommended to confirm antibody specificity. Research utilizing SLC2A4 antibodies has advanced understanding of insulin signaling pathways, adipose-muscle crosstalk in metabolism, and potential drug targets for diabetes. However, challenges remain in standardizing GLUT4 quantification due to its low basal membrane presence and tissue-specific expression patterns.
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