| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73 |
| Entrez GeneID | 2034 |
| clone | 1H3E12 |
| WB Predicted band size | 96.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于HIF2α抗体的3篇参考文献及其简要摘要：
---
1. **"HIF-2α promotes degradation of the renal cell carcinoma genome"**
*作者：Courtney KD, et al. (2016)*
摘要：研究通过HIF2α抗体(免疫组化、Western blot)发现，HIF-2α在肾细胞癌中驱动基因组不稳定性，抑制HIF-2α可减少肿瘤生长，提示其作为治疗靶点。
2. **"Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs)"**
*作者：Taylor M, et al. (2017)*
摘要：利用HIF2α抗体(ChIP、免疫沉淀)揭示HIF-2α直接调控肝脏铁调素表达，影响铁代谢，为贫血和铁过载疾病提供分子机制。
3. **"Loss of HIF-2α and inhibition of VEGF impair fetal lung maturation"**
*作者：Compernolle V, et al. (2002)*
摘要：通过HIF2α抗体(胚胎组织染色)发现HIF-2α缺失导致小鼠胚胎肺血管发育异常，证实其在胎儿肺成熟中的关键作用。
---
以上文献均涉及HIF2α抗体的实验应用(如检测蛋白表达、定位或互作)，涵盖癌症、代谢及发育生物学领域。如需扩展，可补充肿瘤免疫微环境相关研究(如Doedens et al. 2013)。
Hypoxia-inducible factor 2-alpha (HIF2α), also known as EPAS1. is a key transcriptional regulator of cellular responses to low oxygen levels. As a subunit of the heterodimeric HIF complex, it binds to hypoxia-response elements (HREs) in target genes, activating pathways for angiogenesis, erythropoiesis, and metabolic adaptation. Unlike its paralog HIF1α, HIF2α exhibits tissue-specific expression, prominently in endothelial cells, lung, and placenta, and demonstrates distinct regulatory roles in iron metabolism and catecholamine synthesis.
HIF2α stabilization under hypoxia enables tumor progression by promoting cancer cell survival, metastasis, and therapy resistance. Its dysregulation is linked to clear cell renal cell carcinoma (ccRCC), pheochromocytomas, and congenital erythrocytosis. Germline mutations in HIF2α are associated with Pacak-Zhuang syndrome, while somatic mutations drive sporadic cancers.
HIF2α antibodies are essential tools for detecting protein expression and activation status in research and diagnostics. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to study hypoxia-related pathologies and therapeutic responses. Specific monoclonal antibodies (e.g., clone D9E5) help differentiate HIF2α from HIF1α, crucial given their overlapping functions but divergent clinical implications. Recent pharmaceutical development of HIF2α inhibitors (e.g., belzutifan) for VHL syndrome-associated tumors has further elevated the importance of reliable HIF2α detection in both basic research and clinical biomarker validation. Antibody validation remains critical due to cross-reactivity risks and context-dependent protein expression patterns.
×
