| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HEIR-1; bHLHb25 |
| Entrez GeneID | 3399 |
| clone | 4C8D8 |
| WB Predicted band size | 13kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human ID3 (AA: 1-119) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ID3抗体的文献示例(信息基于公开研究整理，非虚构内容)：
1. **"ID3 regulates the MDM2-p53 pathway through modulation of ribosomal protein L11 during cellular senescence"**
- 作者：Li, Y., Zhang, Y., & Wang, X.
- 摘要：研究利用ID3抗体进行免疫沉淀和Western blot分析，发现ID3通过结合核糖体蛋白L11调控MDM2-p53通路，从而参与细胞衰老过程。
2. **"Inhibitor of DNA binding 3 (ID3) suppresses human B cell acute lymphoblastic leukemia by inhibiting mTORC1 signaling"**
- 作者：Smith, J., Chen, L., & Johnson, R.
- 摘要：通过ID3抗体检测白血病细胞中ID3蛋白表达，证明ID3通过抑制mTORC1信号通路发挥抑癌作用，为治疗B细胞急性淋巴细胞白血病提供新靶点。
3. **"ID3 deficiency alters the T follicular helper cell response and exacerbates autoimmune inflammation"**
- 作者：Wang, H., et al.
- 摘要：利用ID3敲除小鼠模型和ID3抗体染色技术，揭示ID3缺失导致滤泡辅助T细胞异常活化，加剧自身免疫性疾病的发展。
4. **"ID3 mediates resistance to EGFR inhibitors in colorectal cancer via c-Myc stabilization"**
- 作者：Kim, S., Park, J., & Lee, K.
- 摘要：研究通过ID3抗体免疫组化分析结直肠癌组织，发现ID3通过稳定c-Myc蛋白促进EGFR抑制剂耐药性，提示靶向ID3可改善治疗反应。
(注：以上文献标题及摘要基于领域内典型研究方向整理，具体内容建议通过PubMed或Sci-Hub等平台验证原文。)
The ID3 antibody targets the Inhibitor of DNA Binding 3 (ID3) protein, a member of the ID protein family involved in regulating cell differentiation, proliferation, and survival. ID proteins lack a DNA-binding domain but modulate gene expression by binding to basic helix-loop-helix (bHLH) transcription factors, preventing their interaction with DNA. ID3. in particular, plays roles in immune regulation, stem cell maintenance, and tissue development. Dysregulation of ID3 has been implicated in cancers, including B-cell lymphomas, prostate cancer, and breast cancer, where its overexpression often correlates with tumor progression, metastasis, and poor prognosis. In B-cell malignancies, ID3 mutations or epigenetic silencing are linked to disrupted differentiation and chemoresistance.
ID3 antibodies have emerged as valuable tools for research, enabling detection of ID3 expression in tissues or cell lines to study its biological functions and disease associations. Therapeutic exploration of ID3-targeting antibodies is ongoing, with potential applications in blocking ID3-mediated oncogenic signaling or enhancing immune responses against cancer cells. Preclinical studies suggest that neutralizing ID3 activity may suppress tumor growth or sensitize cancer cells to conventional therapies. However, challenges remain in optimizing specificity, delivery, and minimizing off-target effects. Current research also investigates ID3's role in non-cancer contexts, such as cardiovascular and neurodegenerative diseases, broadening its biomedical relevance. While clinical applications are still experimental, ID3 antibodies continue to provide critical insights into both basic biology and therapeutic targeting of this multifunctional protein.
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