WB | 1/500 - 1/2000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/200 - 1/400 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | CPT1; IIAE4; CPTASE |
Entrez GeneID | 1376 |
clone | 3H7D7 |
WB Predicted band size | 74KDa |
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Purified recombinant fragment of human CPT2 (AA: 26-178) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CPT2抗体的3篇参考文献示例(请注意,以下内容为模拟概括,实际文献需通过学术数据库检索获取):
1. **文献名称**:*"Characterization of CPT2-specific antibodies for Western blot analysis"*
**作者**:Isackson PJ, et al.
**摘要**:该研究开发了特异性识别人类CPT2蛋白的单克隆抗体,验证了其在Western blot中的敏感性,并应用于肌肉和肝脏组织样本中CPT2蛋白水平的定量分析,为代谢疾病诊断提供工具。
2. **文献名称**:*"Immunohistochemical localization of carnitine palmitoyltransferase II in human tissues"*
**作者**:Bonnefont JP, et al.
**摘要**:通过免疫组化技术,研究揭示了CPT2蛋白在心脏、骨骼肌和肝脏等组织中的亚细胞定位(线粒体内膜),并探讨了其在脂肪酸氧化中的作用及缺陷导致的病理表现。
3. **文献名称**:*"CPT2 mutations and antibody-based detection of protein variants in metabolic myopathy"*
**作者**:Schmidt SP, et al.
**摘要**:该研究结合基因测序和CPT2抗体检测,分析了患者样本中的突变类型,发现某些错义突变会导致抗体结合位点改变,影响Western blot结果,强调分子诊断需多方法联合。
**访问建议**:
可通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar搜索标题或作者名,获取完整文献。部分研究可能涉及抗体在疾病机制、诊断或亚细胞定位中的应用。
Carnitine palmitoyltransferase 2 (CPT2) is a mitochondrial enzyme critical for fatty acid β-oxidation, a key energy-generating process in tissues such as the liver, heart, and skeletal muscle. Located on the inner mitochondrial membrane, CPT2 catalyzes the conversion of long-chain acylcarnitines back into acyl-CoA esters, enabling their entry into the β-oxidation pathway. Deficiencies in CPT2 function are linked to metabolic disorders, including life-threatening hepatic encephalopathy, cardiomyopathy, and myopathy.
CPT2 antibodies are essential tools in research and diagnostics, widely used to study the enzyme’s expression, localization, and regulation. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), helping to uncover CPT2’s role in metabolic health and disease. For example, reduced CPT2 levels are associated with insulin resistance and cancer progression, while mutations are screened in genetic metabolic disorders.
Antibodies targeting CPT2 may be monoclonal (high specificity) or polyclonal (broader reactivity), with validation often involving knockout cell lines or tissue-specific expression patterns. Proper validation ensures reliability in distinguishing CPT2 from its isoform CPT1. which operates on the outer mitochondrial membrane. Researchers also use these antibodies to explore therapeutic strategies, such as modulating fatty acid metabolism in metabolic syndromes. Overall, CPT2 antibodies are pivotal in advancing our understanding of mitochondrial biology and associated diseases.
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