| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDHA; PDHAD; PHE1A; PDHCE1A |
| Entrez GeneID | 5160 |
| clone | 2A2H7 |
| WB Predicted band size | 43.3kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDHA1 (AA: 241-390) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDHA1抗体的3篇示例参考文献(内容为虚构示例，仅供参考)：
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1. **标题**：*PDHA1 Phosphorylation Regulates Metabolic Reprogramming in Cancer Cells*
**作者**：Smith A, et al.
**摘要**：本研究利用PDHA1特异性抗体，通过Western blot和免疫组化技术，揭示了PDHA1在丝氨酸残基的磷酸化如何抑制其酶活性，导致癌细胞中糖代谢向乳酸生成的转变，促进肿瘤生长。
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2. **标题**：*Development of a High-Affinity Monoclonal Antibody for PDHA1 in Human Tissues*
**作者**：Chen L, et al.
**摘要**：作者开发了一种新型PDHA1单克隆抗体，验证了其在免疫沉淀和免疫荧光中的高特异性，并应用于临床样本分析，发现PDHA1表达缺失与线粒体功能障碍疾病相关。
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3. **标题**：*PDHA1 as a Biomarker in Neurodegenerative Disorders: Insights from Antibody-Based Assays*
**作者**：Gomez R, et al.
**摘要**：通过PDHA1抗体对阿尔茨海默病模型脑组织进行蛋白分析，发现PDHA1的异常聚集与线粒体能量代谢缺陷密切相关，提示其作为神经退行性病变潜在生物标志物的价值。
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**注**：以上文献为示例，实际研究中需通过PubMed或Web of Science等平台检索真实文献。
The PDHA1 antibody is a crucial tool for studying the pyruvate dehydrogenase complex (PDC), a mitochondrial enzyme essential for cellular energy metabolism. PDHA1 encodes the E1 alpha subunit of PDC, which catalyzes the conversion of pyruvate to acetyl-CoA, linking glycolysis to the tricarboxylic acid (TCA) cycle. This reaction is tightly regulated by phosphorylation/dephosphorylation mechanisms, with PDHA1 serving as a primary regulatory target. Dysregulation of PDHA1 is associated with metabolic disorders, including pyruvate dehydrogenase deficiency (PDHD), a rare genetic disease causing lactic acidosis and neurological impairments, and cancer, where altered PDHA1 expression may promote the Warburg effect (aerobic glycolysis).
PDHA1 antibodies are widely used in research to investigate metabolic reprogramming in cancer, mitochondrial disorders, and diabetes. They enable the detection of PDHA1 protein levels, phosphorylation status (e.g., at Ser293), and subcellular localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Both monoclonal and polyclonal antibodies are available, often validated in knockout models to ensure specificity. Commercial antibodies typically undergo rigorous testing for cross-reactivity and batch consistency. Recent studies also explore PDHA1's role in immune responses and neurodegenerative diseases, expanding its therapeutic relevance. Proper validation using tissue-specific controls is critical due to potential isoform cross-reactivity or phosphorylation-dependent epitope masking.
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