| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TPS1; TPS2; TPSB1; TPSB2; Tryptase-2 |
| Entrez GeneID | 7177 |
| clone | 1B2E6 |
| WB Predicted band size | 30.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TPSAB1 (AA: 31-275) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"Tryptase genetics and anaphylaxis"**
- **Authors**: Lyons JJ, Sun G, Stone KD
- **摘要**: 该研究探讨了TPSAB1基因拷贝数变异与肥大细胞类胰蛋白酶水平的关系，发现基因剂量效应可能影响过敏反应严重程度及肥大细胞相关疾病的易感性。
2. **"Diagnostic value of tryptase in anaphylaxis and mastocytosis"**
- **Authors**: Valent P, Akin C, Metcalfe DD
- **摘要**: 综述了TPSAB1编码的类胰蛋白酶作为肥大细胞活化生物标志物的临床应用，强调其在系统性肥大细胞增多症和严重过敏反应诊断中的重要性，并讨论了抗体检测的技术进展。
3. **"Heritable dominance gene defects in TPSAB1 cause elevated basal serum tryptase"**
- **Authors**: Lyons JJ, Yu X, Hughes JD, et al.
- **摘要**: 通过家系研究证实TPSAB1基因的显性遗传突变导致血液类胰蛋白酶水平持续升高，提出该基因缺陷与多种肥大细胞介导的疾病相关，为靶向治疗提供理论依据。
4. **"Targeting tryptase in mast cell disorders: a review of current therapeutic strategies"**
- **Authors**: Carter MC, Metcalfe DD, Komarow HD
- **摘要**: 分析了针对TPSAB1表达产物的单克隆抗体及小分子抑制剂在肥大细胞激活综合征治疗中的潜力，总结临床前及早期临床试验结果，强调靶向类胰蛋白酶的治疗前景。
TPSAB1 antibodies target tryptase beta-1. a serine protease primarily expressed in mast cells. Encoded by the *TPSAB1* gene, this enzyme is stored in mast cell granules and released upon activation, playing a key role in allergic responses, inflammation, and tissue remodeling. Tryptase beta-1 cleaves extracellular matrix proteins, activates protease-activated receptors (PARs), and modulates immune cell activity, contributing to conditions like asthma, anaphylaxis, and mastocytosis.
As a diagnostic and research tool, TPSAB1 antibodies are used to detect mast cell density or activation in tissues, aiding in the diagnosis of mast cell disorders (e.g., systemic mastocytosis) or allergic diseases. They also help study mast cell biology, including degranulation mechanisms and pathological roles in chronic inflammation or fibrosis. Commercially available TPSAB1 antibodies are typically validated for techniques like immunohistochemistry, Western blotting, or flow cytometry. Some antibodies distinguish tryptase beta-1 from its isoform, tryptase alpha, due to sequence differences in the C-terminal region. Challenges include cross-reactivity with other tryptases or species-specific epitopes, necessitating careful validation. Research using these antibodies has advanced understanding of mast cell-mediated diseases and therapeutic targeting of tryptase pathways.
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